HPLC法测定痛舒胶囊中三七皂苷R_1及人参皂苷Rg_1、Rb_1

Determination of notoginsenoside R_1 and ginsenoside Rg_1、Rb_1 in Tongshu Capsules by HPLC

目的建立液相色谱法测定痛舒胶囊中三七皂苷R1及人参皂苷Rg1、Rb1含量。方法采用WatresSymmetry RP18(4.6 mm×250 mm,5μm)色谱柱,乙腈-水为流动相,梯度洗脱,体积流量1.0 mL.min-1,检测波长203 nm,柱温30℃。结果三七皂苷R1的线性范围为0.206～3.09μg(r=0.999 4),平均加样回收率为104.2%,RSD为0.77%(n=9);人参皂苷Rg1的线性范围为0.882～13.23μg(r=0.999 5),平均加样回收率为100.7%,RSD为1.1%(n=9);人参皂苷Rb1的线性范围为0.921～13.82μg(r=0.999 2),平均加样回收率为103.1%,RSD为2.1%(n=9)。结论本方法灵敏、准确,重现性好,可用于本制剂的含量测定及质量控制。

Objective To establish a method for determination of notoginsenoside Rj and ginsenoside Rg1 , Rbl in Tongshu Capsules by HPLC. Methods Waters Symmetry RPjs （4.6 mm x 250 mm,5 μm ） column was used, with mixture liquid of acetonitrile and water as mobile phase in a gradient mode. The detection wavelength was 203 nm, the column tem- perature was 30 ~C ,and the flow rate was 1.0 mL ~ min-l. Results The linear ranges of notoginsenoside Rl and ginsen- oside Rg~, Rb1 were 0. 206 ~ 3.09 i.Lg （ r = 0.999 4 } ,0. 882 - 13.23 Ixg （ r = 0.999 5 }, 0.921 13.82 μg { r = 0. 999 2 }, the average recoveries（ n =9）were 104.2% IRSD ：0. 77% }, 100.7% [RSD = 1. 1%/, 103.1% （2.1%） ,respectively. Conclusion The method was sensitive, accurate. It can be used for determination and quality control of Tongshu Capsules.