摘要
用诺维信LYN05024液态酶在未添加水和有机溶剂的条件下水解大豆浓缩磷脂,通过响应面试验,获得了酶解大豆浓缩磷脂的最佳条件为:反应温度68℃,时间为1.1 h,加酶量为7.5%.此时磷脂的酸价从17.2 mg/g上升至38.58 mg/g.通过高效液相色谱法测定磷脂的组成,按PC反应前后相对含量的变化,得出酶解率为77%.
Condensed soybean phosphlipids was hydrolyzed by using liquid enzyme Novozymes LYN05024 without adding water and organic solvents. The optimum conditions were determined by response surface methodology as follows： reaction temperature 68 ℃, reaction time 1.1 hours and enzyme amount 7.5%. The acid value of the phospholipids increased from 17.2 mg/g to 38.58 mg/g after modification. The composition of phospholipids was determined by high performance liquid chromatography （HPLC）, and the hydrolysis rate of condensed soybean phosphlipids was 77% after comparing the relative contents of condensed soybean phospholipids before and after the PC reaction.
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2013年第1期36-40,共5页
Journal of Henan University of Technology：Natural Science Edition
关键词
浓缩磷脂
酶改性
酸价
condensed soybean phospholipids
enzymatic modification
acid value
