葡萄原花青素体内外抑制乙型肝炎病毒表面抗原和e抗原的初步研究

Inhibition of grape procyanidins on HBsAg and HBeAg in vitro and in vivo

目的探讨葡萄原花青素体内外对乙型肝炎病毒表面抗原(HBsAg)和e抗原(HBeAg)的抑制作用。方法体外分别取不同浓度(50mg/L、100mg/L和200mg/L)的葡萄原花青素各0.25ml,以1∶1的比例与HBeAg阳性慢性乙型肝炎患者血清共孵育4h后检测其HBsAg和HBeAg的含量,并以生理盐水孵育组作为对照组。体内研究选择60例HBeAg阳性慢性乙型肝炎患者随机分为阿德福韦酯单药治疗组(对照组)和阿德福韦酯联合葡萄原花青素治疗组(试验组),每组各30例患者(其中对照组失访1例)。阿德福韦酯剂量为10mg/d,葡萄原花青素剂量为100mg/次,3次/d,疗程均为8周。治疗前后分别检测其血清HBsAg、HBeAg、HBVDNA水平以及患者的肝功能,同时观察各组患者不良反应情况。结果体外3个浓度葡萄原花青素组与HBeAg阳性血清共孵育后HBsAg和HBeAg含量均较对照组降低(P<0.05)。体内研究中,两组患者治疗后HBsAg水平差异无统计学意义(P>0.05),但试验组患者治疗后HBeAg定量水平低于治疗前和对照组治疗后的水平(P<0.05)。结论葡萄原花青素对HBsAg和HBeAg具有一定程度的抑制作用,值得进一步深入研究。

Objective To observe the inhibition of grape procyanidins on hepatitis B virus surface antigen （HBsAg） and e antigen （HBeAg） in vitro and in vivo. Methods In vitro, 0. 25 ml grape procyanidins in different concentrations （50 mg/L, 100 mg/L and 200 rag/L） were incubated with the serums from HBeAg-positive patients with chronic hepatitis B （CHB） with the ratio of 1 ： 1 for 4 hours, and then to detect the levels of HBsAg and HBeAg in serum of each group. The physiological saline group was taken as the control group. In vivo, sixty cases of HBeAg positive CHB patients were randomly divided into the control group who were treated with adefovir dipivoxil （ADV） alone （n = 30, but one case was failed to be followed-up） and the treatment group who were treated with ADV and grape procyanidins （n = 30）. The ADV dose was 10 mg/d and the grape procyanidins dose was 100 rag, three times per day. The treatment period of both groups were 8 weeks. The serum levels of HBsAg, HBeAg, HBV DNA and the liver function were detected before and after the treatment. The side effect of treatments were observed simuhaneously. Results The serum levels of HBsAg and HBeAg after incubated with the three concentrations of grape procyanidins were significantly lower than that in control group in vitro （ P 〈 O. 05 ）. In vivo, there were no significant difference between the levels of HBsAg before and after treatment in the two groups （ P 〉 0.05 ）. But the serum levels of HBeAg after treatment in treatment group were significantly lower than that before treatment in treatment group and after treatment in control group （P 〈 0. 05 ）. Conclusions Grape procyanidins has a certain degree of inhibition on HBsAg and HBeAg and is worthy for further study.