摘要
目的:研究槲皮素(quercetin)是否对过氧化氢所致PC-12细胞氧化损伤具有保护作用,以及可能的保护机制。方法:用PC-12细胞建立H2O2氧化损伤模型;测定SOD、T-AOC生化指标判断细胞抗氧化能力;半定量RT-PCR法检测糖皮质激素受体(GR)基因转录水平。结果:①MTT结果:H2O2能使细胞活力显著降低(P<0.01),槲皮素的预孵处理能够明显减轻H2O2对PC-12细胞的氧化损伤(P<0.05&0.01)。②SOD活性结果:H2O2作为氧化损伤因素使得细胞SOD活性应激上升(P<0.01),Qu组细胞SOD活性维持在较低水平(P<0.01)。③T-AOC结果:槲皮素能够显著提高PC-12细胞的总抗氧化能力(P<0.01)。④半定量RT-PCR结果:H2O2使PC-12细胞GR基因转录水平显著降低,槲皮素能够减轻其转录所受影响(P<0.01)。结论:槲皮素与PC-12细胞的共孵育,提高了细胞整体的抗氧化能力,维持了GR基因的转录水平,继而保护细胞免受后续H2O2的氧化损伤和炎症反应,维持细胞生化环境的稳态。
Objective: To investigate the protective effects of quercetin on PC-12 cells against hydrogen peroxide-induced oxidative damage and protective mechanisms.Methods: The oxidative damage model of PC-12 cells was established by treated with H2O2.The viability of PC-12 cells was determined by MTT assay.The antioxidative capacity was judged by SOD and T-AOC determinations.The transcriptional level of glucocorticoid receptor gene was detected by semi-quantitative RT-PCR method.Results: ① Cell Viability: H2O2 group was lower than that in the Control group(P0.01),while Qu groups were higher than that in the H2O2group(P0.05 0.01).② SOD Activity: H2O2group was higher than that in the Control group(P0.01),while Qu groups were lower than that in the H2O2group(P0.01).③ResultsofT-AOC:ThevaluesofQugroupswerehigherthanthatintheControlgroup(P0.01).④ResultsofSemi-quantitative RT-PCR: H2O2reduced cells' GR gene transcriptional level significantly,while quercetin could relieve the impact(P0.01).Conclusions: The incubation with quercetin could improve the antioxidative capacity of PC-12 cells,preserve the GR gene transcriptional level,and then protect cells from H2O2induced oxidative damage and inflammation,thereby maintain cellular and biochemical homeostasis.
出处
《现代生物医学进展》
CAS
2012年第35期6801-6804,6818,共5页
Progress in Modern Biomedicine
基金
深圳市国际科技合作项目(ZYA200903270135A)