灌流速度与新西兰兔心肌细胞急性分离的关系(英文)

Relation between perfusion speed and acute separation of myocardial cells in New Zealand rabbits

目的:建立一种简单,稳定,成功率高的新西兰兔心肌细胞分离方法。方法:比较分离细胞过程中Langen-dorff灌流装置的灌流速度[50r/min(8ml/min)和65r/min(11ml/min)]对细胞分离的影响。结果:与灌流速度为50r/min时比较,灌流速度为65r/min获得细胞所需时间[(33±9.10)min比(19.29±2.73)min]明显缩短,成功率(50%比91%)、存活率[(38.26±2.63)%比(85.71±2.21)%]明显提高(P均<0.01)。结论:灌流速度是影响新西兰兔心肌细胞急性分离的重要因素,65r/min灌流速度能更稳定、成功获取单个心肌细胞。

Objective.. To establish a simple, stable and high success rate separation method of myocardial cells in New Zealand rabbits. Methods： Compared influence between perfusion speed of 50 r/min （8 ml/min） and 65 r/min （11 ml/min） of Langendorff perfusion device on cell separation during separation course of myocardial cells. Results.. Compared with perfusion speed of 50 r/min, there was significant decrease in time to obtain cells [ （33±9.10） min vs. （19.29± 2.73） mini, and significant increase in success rate （50% vs. 91%） and survival rate F （38.26 ± 2.63）% vs. （85.71±2.21）%] by perfusion speed of 65 r/min, P〈0.01 all. Conclusion： Perfusion speed is an im- portant factor that affects acute separation of myocardial cells in New Zealand rabbits, and 65r/min perfusion speed may be more stable and successful to obtain single myocardial cell.