外源性磷酸肌酸对力竭运动小鼠的影响

Effect of exogenous phosphocreatine on exhaustive exercise mice

目的:观察外源性磷酸肌酸对一次性力竭运动小鼠的影响,探讨其抗疲劳的作用机制。方法:采用小鼠负重游泳建立力竭运动小鼠模型。将小鼠分为力竭给药组(A组)、力竭不给药组(B组)、游泳8min不给药组(C组)、游泳8min给药组(D组)。实验前20min,A、D组小鼠按1000mg/Kg体质量腹腔注射磷酸肌酸钠,B、C组腹腔注射同等剂量的生理盐水。力竭游泳实验按每只小鼠体质量的6%负重进行。用生化比色法检测血清超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,并记录小鼠的力竭时间。结果:A、C、D组的SOD活性较B组显著升高(P<0.05,0.01),D组较A组明显升高(P<0.05)。B组的MDA含量较C组明显升高(P<0.01),A组较D组明显升高(P<0.05)。B组游泳至力竭的时间明显短于A组(P<0.05)。结论:外源性磷酸肌酸增强机体抗疲劳的机制可能与其增强SOD活性、降低MDA含量有关,通过增强自由基的清除来提高机体的抗疲劳能力。

Objective：To observe the influence of exogenous phosphocreatine on exhaustive exercise mice,and research the mechanism of antifatigue. Methods：The method of mice weight loading swimming was used to set up the model of exhaustive exercise. The mice were divided into group of exhaustion with medicine as group A,group of exhaustion without medicine as group B, group of swimming for 8 min without medicine as group C, and group of swimming for 8 min with medicine as group D. Twenty rain before the experiment,mice in the groups A and D were intraperitoneally injected with creatine phosphate sodium by the standard of 1000mg/kg,and those in the groups B and C were intraperitoneally injected with the same wolume of normal saline. The exhaustive swimming experiment was conducted on the loading weight with 6% of the weight of each mouse. Serum SOl） activity and MDA content were determined by using biochemistry colorimetry,and the exhaustive time was recorded. Results：The SOD activity in groups A,C and D was stronger than in group B （P〈0.05,0.01） ,and stronger in group D than in group A（P〈0.05）. The MDA content in group B was higher than in group C（P〈0.01） ,and higher in group A than in group D （P〈0.05）. The time from swimming to exhaustion in group 13 was significantly shorter than in group A（P〈0.05）. Conclusion：The mechanism by which exogenous phosphocreatine strengthens the ability of organisms to fight against fatigue may be related to the enhancement of SOD activity and reduction of MDA content,which improves the organism＇s ability to resist fatigue by enhancing the removal of the free radicals.