n-6／n-3多不饱和脂肪酸对子宫内膜癌Ishikawa细胞增殖的影响

The effects and mechanisms of different n -6/n -3 polyunsaturated fatty acid ratio on endometrial carcinoma cells

目的探讨n-6／n-3多不饱和脂肪酸（PUFAs）对子宫内膜癌Ishikawa细胞增殖及细胞中哺乳动物雷帕霉素靶蛋白（mammMiantargetofrapamycin，mTOR）、血管内皮生长因子（vascularendothelialgrowthfactor，VEGF）表达的影响。方法用不同比例的n-6／n-3PUFAs处理细胞，MTr法检测细胞增殖能力变化；RT—PCR检测细胞mTOR、VEGF的mRNA表达情况。结果单纯n-6PUFAs和10：1n-6／n-3PUFAs能够明显促进Ishikawa细胞增殖，mTOR、VEGF的mRNA表达增加（P〈0．05）；单纯n-3PUFAs和1：1n-6／n-3PUFAs则显著抑制细胞增殖，同时使VEGF的mRNA表达下降（P〈0．05）；mTOR与VEGFmRNA的表达呈正相关（r=0．94，P〈0．05）。结论n-6／n-3PUFAs可能通过影响mTOR信号调节VEGF表达，从而对Ishikawa细胞增殖产生影响。

Objective To explore the effects and mechanisms of different n - 6/n - 3 polyunsaturated fatty acids （PUFAs） ratio on endometrial carcinoma Ishikawa cells. Methods Ishikawa cells were treated with n -6 PUFAs, or n - 3 PUFAs, or the mixture of n - 6/n - 3 PUFAs at ratio of 1 ： 1 and 10：1. The cell proliferation capacity was detected by MTF, and the expressions of mTOR, VEGF mRNA were tested by RT - PCR respectively. Results n - 6 PUFAs and 10：1 n -6/n -3 PUFAs obviously promoted cell proliferation and up -regulated the expression of mTOR,VEGF mRNA. n- 3 PUFAs and 1：1 n -6/n- 3 PUFAs greatly repressed cell proliferation and down -regulated roTOR, VEGF mRNA expression. There was significant correlation between mTOR and VEGF in Ishikawa cell （ r = 0.94, P 〈 O. 05 ）. Conclu- sion n -6/n -3 PUFAs at different ratios has different effects in endometrial carcinoma Ishikawa cells through working on mTOR signal to regulate VEGF expression probably.