16种脑胶质瘤动物模型的N—cadherin肽链差异性研究

The difference of polypeptide chains of N- cadherin among 16 animal models of glioma

目的探讨不同动物模型与人的神经钙黏素（N—cadherin）肽链之间的差异，从而评估这些动物作为胶质瘤动物模型的可行性。方法采用Clustalx1．83和MEGA4．0软件对16种动物的N—cadherin肽链进行最大似然分析。结果得到不同种间N—cadherin成熟肽链的氨基酸变化位点和1棵最大似然合意树。结论作为细胞表面重要的黏附分子，N—cadherin在肿瘤转移与入侵过程中起着关键性作用，因此构建胶质瘤动物模型时，有必要使所采用的动物模型的N—cadherin肽链尽可能与人类的一致。

Objective To evaluate the feasibility of animal models of glioma according to the difference of polypep- tide chains of N - cadherin among these animals and human. Methods Maximum likelihood analysis was performed with amino acids sequences of 16 N - cadherin using Clustalx 1.83 and MEGA 4.0 softwares. Results Varied amino acid sites among polypeptide chains of N - eadherin of 16 animals and a maximum likelihood tree were obtainted. Conclusion As an important adhesive molecule, N - cadherin is a key protein for metastasis and invasion of the glioma cells. When animal models of glioma are planned to be established, their amino acid sequences of N - cadherin should be as consistent as possible with those of human tissues.