补肾填精法对兔骨折后不同时相骨髓间充质干细胞成骨分化的影响

Effects of Traditional Chinese Medicine Formula for Nourishing Kidney-Essence on Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in Rabbits in Different Phases of Fracture Healing

目的观察补肾填精中药对兔骨折后不同时点骨髓间充质干细胞(bone marrow mesenchymal stemcells,BMSCs)分化为成骨细胞的影响。方法将20只白兔随机分成4组,即正常对照组、骨折模型组、中药治疗组、西药治疗组。分离培养骨折端BMSCs,取P3代BMSCs并向成骨细胞诱导分化,分别采用碱性磷酸酶(alkaline phosphatase,ALP)试剂盒、骨钙素(osteocalcin,OCN)试剂盒检测ALP活性、OCN水平。结果随着用药时间的延长,各骨折组ALP活性和OCN水平均逐渐增强。在骨折后第14、21天,中药治疗组ALP活性、OCN水平均高于其他3组。结论补肾填精法可促进兔骨折后不同时点BMSCs向成骨细胞分化。

Objective To observe the effects of traditional Chinese medicine （TCM） formula for nourishing kidney-essence on the osteogenie differentiation of bone marrow mesenchymal stem cells （BMSCs） in rab- bits in different phases of fracture healing. Methods Twenty white rabbits were divided into normal con- trol group, fracture model group, TCM treatment group, and Western medicine （WM） treatment group. The BMSCs at the fracture ends were isolated and cultured, and the passage 3 cells were collected and then induced to differentiate into osteoblasts. Alkaline phosphatase （ALP） assay kit and osteocalcin （OCN） as- say kit were used to measure the ALP activity and OCN level,respectively. Results After fracture, the ALP activity and OCN level increased gradually in the fracture model group, TCM treatment group, and WM treatment group. At 14 and 21 days after fracture, the TCM treatment group had significantly higher ALP activity and OCN level than the other three groups （P（0.05 or P（0.01）. Conclusion TCM formu- la for nourishing kidney-essence can promote the osteogenic differentiation of BMSCs in rabbits in different phases of fracture healing.