加味犀角地黄汤在脂多糖诱导的兔内毒素血症中的防治作用

Effects of Modified Xijiao Dihuang Decoction in Rabbit Model of Endotoxemia Induced by Lipopolysaccharide

目的:通过比较加味犀角地黄汤与桃红四物汤对细菌脂多糖诱导的兔内毒素血症中ET-1、vWF、TNF-α、CD62P表达及球结膜微循环的影响,评价凉血化瘀法在内毒素血症中的治疗作用。方法:将32只新西兰大白兔按随机分为正常对照组、模型对照组、活血组、凉血组,每组各8只。活血组及凉血组采用脂多糖诱导内毒素血症并分别给以桃红四物汤及加味犀角地黄汤灌胃。治疗中注射脂多糖后分别于3 h、24 h、7 d时ELISA检测兔血清ET-1、vWF、TNF-α水平,细胞流式法检测CD62P变化,记录球结膜微循环图像。结果:凉血组、活血组及模型对照组在注射脂多糖后3 h兔血清ET-1、vWF、TNF-α、CD62P水平及球结膜微循环积分明显高于正常对照组(P<0.05-0.01),注射后3 h及24 h时,凉血组所有观察指标及活血组除TNF-α外的其他指标与模型对照组相比均有明显下降(P<0.05)。各时间点凉血组各指标下降较活血组更为明显。结论:活血化瘀在脂多糖诱导的内毒素血症中具有抗炎、抑制血小板活化、防治内皮损伤及改善微循环等作用,加味犀角地黄汤具有更强抗炎作用。

Objective ： To investigate the effects of promoting blood circulation and removing blood stasis on expressions of ser- um ET- 1 ,vWF, TNF-a and CD62P levels and conjunctiva microcirculation disorders in rabbit model of endotoxemia induced by lipopolysaceharide. Methods ：32 New Zealand white rabbits were randQmized into 4 groups for blood-cooling group, blood -pro- moting group,model control group and normal control group. Treated with modified X^iao Dihuang decoction and Taohong Siwu decoction respectively while modeling, serum CD62P was determined by flow cytometry and serum ET - 1, vWF and TNF - a lev- els were determined by ELISA. Conjunctiva microcirculation changes were recorded. Results ： At 3rd hour after injection of LPS, the levels of serum ET- 1 ,vWF, CD62P and TNF-a were getting higher significantly in three groups than those in normal control group（P〈0. 05 ,P 〈0. 01）. At 3rd and 24th hour after injection,serum ET - 1 ,vWF,TNF - ct and CD62P levels and scores of microcirculation in blood-cooling group and ET - 1, vWF, CD62P and scores of microcirculation in blood - promoting group were significantly lower than model control group （P 〈 0.05 ）. Conclusion：The therapy of promoting blood circulation and removing blood stasis has efficacy of anti - inflammation,suppressing activation of platelet and protection against vascular endothelial inju- ry. Modified Xijiao Dihuang deegction is more effective than Taohong Siwu decoction in rabbit model of endotoxemia induced by lipopolysaceharide.