针刺保肝护脑对脑缺血再灌注 肝损伤大鼠CATmRNA表达的影响

Effect on CATmRNA Expression in Cerebral Ischemia Reperfusion Rat with Hepatic Injury Treated by Protecting Liver to Nourish Brain Therapy

目的:探讨针刺保肝护脑对脑缺血再灌注肝损伤大鼠CATmRNA表达的影响。方法:将40只SD雄性大鼠随机分为假手术组、模型组、针刺对照组、针刺保肝护脑组。线栓法制作缺血再灌注大鼠模型,测试大鼠行为学、血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、过氧化氢酶(CAT)活性、肝组织CAT mRNA含量。结果:模型组、针刺对照组和针刺保肝护脑组缺血2 h(电针治疗前)时神经功能缺损评分无显著差异;经10次电针治疗结束后:与模型组相比,针刺对照组和针刺保肝护脑组神经功能缺损评分有极显著差异(P<0.01);与针刺对照组相比,针刺保肝护脑组神经功能缺损评分变化更为显著(P<0.05)。电针治疗前,与假手术组、针刺对照组和针刺保肝护脑组比较,模型组血清ALT、AST水平显著增高(P<0.01),而血清CAT、肝组织CATmRNA明显降低(P<0.01);经10次电针治疗结束后:与模型组相比,针刺对照组和保肝护脑针法组血清ALT、AST水平明显降低(P<0.01),同时血清CAT、肝组织CATmRNA显著升高(P<0.01);与针刺对照组相比,针刺保肝护脑组血清ALT、AST、CAT和肝组织CAT mRNA各项变化更为显著(P<0.05)。结论:针刺保肝护脑对脑缺血再灌注大鼠的治疗效果较单纯头穴针刺更为显著,其治疗机理与促进肝脏CATmRNA表达相关。

Objective ： To explore the effect of protecting liver to nourish brain therapy on CATmRNA expression of cerebral is- chemic reperfusion（CIR） rats with hepatic injury. Methods ：40 Sprague Dawley rats were randomly divided into sham operation, CIR model,acupuncture control,protecting liver to nourish brain therapy（PLNB） groups, each with 10 rats. CIR models were es- tablished by right middle cerebral artery occlusion （MCAO）. The neurological deficit scores were evaluated. The serum levels of ALT, AST, CAT （catalase） and hepatic CATmRNA were detected separately by biochemical methods. Results ： Before treatment, the neurological deficit scores had no differences among CIR model, acupuncture control, PLNB groups. After treatment, in comparison with CIR model group, significant differences of the neurological deficit scores were found in acupuncture control and PLNB groups （ P 〈0. 01 ）. There were more significant changes of the neurological deficit scores in PLNB group compared with the acupuncture control one （ P 〈 0. 05 ）. Before treatment, in comparison with sham operation, acupuncture control, PLNB groups, the levels of ser- um ALT and AST were higher but the level of serum CAT and hepatic CAT mRNA were lower in CIR model group（P 〈0. 01 ）. After treatment,in comparison with CIR model group, significant differences of the levels of serum ALT, AST , CAT and hepatic CATmRNA were found in acupuncture control and PLNB groups（P 〈 0. 01 ）. There were more significant changes of serum levels of ALT, AST , CAT and hepatic CATmRNA in PLNB group compared with the acupuncture control one （ P 〈 O. 05 ）. Conclusion ： The protecting liver to nourish brain therapy had more effects on CIR rats than simPle scalp needling, its therapeutical effects are closely related to expression of hepatic CATmRNA.