外寒对小鼠肺组织BAFF和NF-κB表达的影响

Effects of External Cold on Expressions of BAFF and NF-κB in Lung of Mice

目的:观察外寒对小鼠肺组织BAFF和NF-κB表达的影响。方法:50只昆明小鼠随机分为5组:常温对照组、4℃7天组、4℃14天组、0℃7天组、0℃14天组,置于人工气候箱中处理后取小鼠肺脏进行病理切片,ELISA法检测IgG-S和IgG-R,实时荧光定量PCR检测BAFF的表达,Western blot法检测小鼠肺组织中NF-κB蛋白的表达情况。结果 4℃7天组、0℃7天组小鼠肺部出现明显的病理的改变,肺泡内出现大量的渗出液,各实验处理组IgG-S和IgG-R较常温对照组明显升高,差异具有显著性意义,实时荧光定量PCR检测发现各处理组BAFF的表达与常温对照组比较,明显上调,Western blot结果显示各处理组NF-κB蛋白的表达较常温对照组明显增加。结论外寒通过上调BAFF和NF-κB的表达,促发机会的获得性免疫。

Objective：To observe the effects of external cold on the BAFF and NF - KB expressions in lung tissue of mice. Methods：50 Kunming mice were divided into five groups：normal group,4℃/7d group,4℃/14d group,0℃/7d group and 0℃/ 14d group. Mice were put into different temperature - controlled boxes, then samples were collected to observe their pathological changes in pulmonary tissue, IgG - S and IgG - R were measured with ELISA. The expression of BAFF was detected by real time PCR, and NF - KB was detected by western blot. Results：Compared with the normal group, the 4℃/7d group and 0℃/7d group pulmonary tissues had obvious changes, alveoli edema could be observed. IgG - S and IgG - R of the 4℃/7d group ,4℃/14d group,0℃/7d group and 0℃/14d group were higher（ P 〈 0. 05 ）. The BAFF and NF- KB expressions of 4℃/7d group and 0℃/7d group were higher than that of the normal group. Conclusion：The external cold impaired lung,the defense function of lung was damaged and caused the pulmonary infection,which induced the acquired immunity and made the expressions of BAFF and NF - KB increase.