坎离颗粒对腹主动脉缩窄致慢性心衰大鼠骨骼肌细胞凋亡的影响

Effects of “Kanli Granule” on Skeletal Muscle Apoptosis in Rats with Chronic Heart Failure Induced by Constriction of Abdominal Aorta

目的:观察中药坎离颗粒对腹主动脉缩窄致慢性心衰大鼠骨骼肌细胞凋亡的影响。方法:以腹主动脉缩窄致慢性心衰大鼠为研究对象,对其细胞凋亡基因Bax、Bcl-2的蛋白表达进行观察,采用TUNEL检测凋亡细胞;并分别以中药坎离颗粒生药高(13.5 g/kg、)、中(6.75 g/kg)、低(3.375 g/kg)剂量及培哚普利(0.36 mg/kg)每日1次灌胃,3周1疗程,每疗程后停药1周,共干预8个疗程。结果:慢性心衰大鼠存在骨骼肌细胞凋亡增多的现象;培哚普利、坎离颗粒预防给药能改善上述病理变化,并观察到坎离颗粒改善心衰时骨骼肌细胞凋亡呈现明显的剂量依赖关系。结论:坎离颗粒在改善腹主动脉缩窄致慢性心衰大鼠心功能的同时还能改善其细胞凋亡,对骨骼肌功能的改善可能起有益作用。

Objective： To investigate the effects of ”Kanli Granule“ on the skeletal muscle apoptosis in rats with chronic heart failure （CHF） induced by constriction of abdominal aorta. Methods： The CHF rat model was established by ligating the abdominal aorta. The CHF rats were fed intragastrieally with ”Kanli Granule” （ 13.5,6.75, 3. 375 g/kg） and perindopril once a day for 32 weeks. The protein expressions of Bax and Bcl-2 genes were observed, and the apoptosis was detected by TUNEL. Results： The skeletal muscle apoptosis of the CHF rats was increased. Preventive therapy with “Kanli Granule” or perindopril could improve the skeletal muscle apoptosis of the CHF rats, and “Kanli Granule” had a clear dose-dependent relationship in improving the skeletal muscle apoptosis of CHF rats. Conclusion： “Kanli Granule” can improve the skeletal muscle apoptosis in rats with CHF induced by constriction of abdominal aorta.