加味四君子汤对肌萎缩侧索硬化症AR2小鼠运动功能的影响

Effects of “Jiawei Sijunzi Decoction” on Movement Dysfunction of AR2 Mice with Amyotrophic Lateral Sclerosis

目的:探索加味四君子汤对肌萎缩侧索硬化症(ALS)AR2小鼠运动功能的影响。方法:采用转基因方法制作保留部分运动功能的ADAR2flox/flox小鼠为对照组(Ⅰ组),将ALS AR2小鼠随机分为空白组(Ⅱ组)、中药低剂量组(Ⅲ组)及高剂量组(Ⅳ组)。各组小鼠正常进食水,中药组分别给予灌胃低、高剂量的加味四君子汤,每日1次。每4周观察小鼠活动状况以及抓握力、爬滚筒能力等运动功能,并在第24周检测小鼠血常规、心肝肾功能。结果:各组小鼠第8周开始均有不同程度的运动功能减退,到24周为止Ⅰ组运动功能减退速度缓慢,Ⅱ组AR2小鼠从12周开始无论活动度、抓握力还是爬滚筒能力均迅速下降,而AR2小鼠中药治疗组运动功能降低程度被延缓,且与中药剂量有关;24周时Ⅱ组AR小鼠的体质量、抓握力和爬滚筒能力与其余3组比较明显降低,差异有统计学意义。各组小鼠24周时的生化检测均无异常。结论:加味四君子汤能够延缓ALS小鼠运动功能的衰退。

Objective： To investigate the effects of “Jiawei Sijunzi Decoction” on movement dysfunction of AR2 mice with amyotrophic lateral sclerosis （ALS）. Methods： The ADAR2 flox/flox mouse model which remained part of motor functions was established by transgenic methods, and was taken as the control group （ group I ）. The ALS AR2 mice were randomly divided into blank group （ group Ⅱ ） , low dose of Chinese medicine group （ group Ⅲ ） and high dose of Chinese medicine group （group IV ）. All the mice were given normal food and water, and the mice in group m and group IV were fed intragastrically by low dose and high dose “Jiawei Sijunzi Decoction”respectively once a day. The active situation, strength of grip and other movement functions were observed every 4 weeks, and the routine blood and heart, liver and kidney functions were detected at the 24th week. Results： All the mice began to appear different degrees of motion hypofunction from the 8th week, the progress of motion hypofunction was the lowest in group I till the 24th week, the motion functions of mice in group Ⅱ decreased quickly, and the motion functions of mice in group m and group IV were relieved related to the dose of the Chinese medicine. Compared with the other groups, the body weight and motion functions of AR mice in group Ⅱ significantly decreased with statistical difference at the 24th week. There were no differences on biochemical indexes among all the groups. Conclusion： “Jiawei Sijunzi Decoction”can delay the motion hypofunction of ALS mice.