小叶锦鸡儿总黄酮提取工艺研究及对脑缺血再灌注损伤大鼠的作用

Extraction technology of total flavonoid from Caragana microphylla and its effect on cerebral ischemia-reperfusion injury in rats

目的优化小叶锦鸡儿黄酮类成分的提取工艺,探讨其对实验性脑缺血再灌注损伤大鼠的保护作用。方法采用正交试验,以芦丁作为对照,以总黄酮量为指标考察小叶锦鸡儿总黄酮的最佳提取工艺;采用大脑中动脉阻断(MCAO)制备大鼠局灶性脑缺血再灌注模型,ig给药4周后,观察小叶锦鸡儿总黄酮对MCAO模型大鼠学习记忆能力的影响,并测定其血流变学各项指标。结果正交实验结果表明,各因素作用影响主次顺序为:液固比>乙醇浓度>浸提时间>浸提温度。小叶锦鸡儿总黄酮能提高MCAO模型大鼠的学习记忆能力,降低血浆黏度(η)、血小板聚集率(PAgT)、红细胞聚集指数(EAI)、红细胞刚性指数(IR),并增加红细胞变形指数(DI)。结论确定了总黄酮最佳提取工艺为乙醇浓度60%,浸提时间5h,液固比40:1,浸提温度60℃。小叶锦鸡儿总黄酮对脑缺血再灌注损伤具有脑保护作用,这可能与小叶锦鸡儿总黄酮降低血黏度有关。

Objective To optimize the extraction technology of total flavonoid from Caragana microphylla（TFCM） and to explore the protective effect on cerebral ischemia-reperfusion injury in rats.Methods The optimum extraction technology was detected by orthogonal test with rutin as control and with the content of TFCM as an indicator.The middle-cerebral-artery-occlusion（MCAO） was used to induce the focal cerebral ischemia-reperfusion injury model of rats.The influence on the ability of learning and remembering and the effect on hemorheology were observed after ig administration with TFCM for four weeks.Results The result of orthogonal experiment indicated that the order of factors which affected the extraction was as follows：liquid-solid ratio concentration of ethanol extracting time extracting temperature.TFCM could improve the ability of learning and remembering,decrease the levels of blood viscosity（η）,platelet aggregation rate（PAgT）,erythrocyte aggregation index（EAI）,and erythrocyte rigidity index（IR）,and increase the level of erythrocyte deformation index（DI）.Conclusion The optimum extraction conditions are as follows：extracting for 5 h at 60 ℃ with the ratio of 60% ethanol to medicinal herbs（40：1）.TFCM could protect the brain of the model rat with focal cerebral ischemia-reperfusion;It could be relevant to the role of decreasing blood viscosity.