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TNF-α诱导的衰老血管内皮细胞中泛素、细胞周期的变化及意义 被引量：1

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摘要 目的探讨TNF-α诱导的衰老血管内皮细胞中泛素、细胞周期的变化及意义。方法体外培养血管内皮细胞,应用TNF-α诱导血管内皮细胞衰老模型。取传2代细胞,分成空白对照组、实验组,应用RT-PCR法检测细胞内泛素的表达,应用流式细胞术检测细胞周期的变化。结果实验组血管内皮细胞泛素mRNA较空白对照组明显增高(P<0.05),且实验组细胞周期趋向停滞于G0/G1期。结论衰老血管内皮细胞中泛素表达增加,细胞周期停滞,细胞分裂增生减慢。
作者 赵海梅 谭翌 杨小端
机构地区 南昌大学第三附属医院
出处 《山东医药》 CAS 2013年第5期25-27,共3页 Shandong Medical Journal
关键词 细胞凋亡 内皮细胞 泛素 细胞周期
分类号 R329.28 [医药卫生—人体解剖和组织胚胎学]
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参考文献10

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山东医药
2013年 第5期

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