亚低温联合依达拉奉对大鼠脑缺血再灌注损伤的保护作用

Mild hypothermia combined with edaravone on rat with cerebral ischemia reperfusion injury

目的观察亚低温联合依达拉奉对大鼠脑缺血再灌注损伤的保护作用。方法制备SD大鼠MCAO模型,梗死2h后再灌注,并分为假手术组、模型组、依达拉奉组及亚低温联合依达拉奉组(联合组),再灌注24 h后进行神经功能缺损评分、检测脑组织超氧化物歧化酶(SOD)、丙二醛(MDA)浓度、病理变化及大脑缺血面积并比较。结果 (1)依达拉奉组与联合组的神经功能缺损评分明显低于模型组,且联合组的神经功能缺损评分明显低于依达拉奉组(P均<0.01)。(2)与假手术组相比,模型组SOD活性降低、MDA含量增加(P<0.01);与模型组相比,依达拉奉组与联合组S0D活性增高、MDA含量降低(P<0.01),且联合组与依达拉奉组相比有显著性差异(P<0.01)。(3)模型组、依达拉奉组及联合组病理学检查均较假手术组差,但联合组好于依达拉奉组好于模型组(P<0.01)。(4)模型组、依达拉奉组及联合组脑缺血面积较假手术组增大,而联合组<依达拉奉组<模型组,差异均有统计学意义(P<0.01)。结论亚低温联合依达拉奉对脑缺血再灌注损伤有较好保护作用。

Objective To observe mild hypothermia combined with edaravone on rat with cerebral ischemia reperfusion injury.Methods Preparation of SD rat model of MCAO,reperfusion after inlarcted for 2h.They were divided into sham operation group,normal saline（NS） group,edaravone group and mild hypothermia combined with edaravone group,24 h after reperfusion nerve function deficit score,detection of brain tissue SOD enzyme（SOD）,malondialdehyde（MDA） concentration, pathologic picture and brain ischemia area are compared.Results（1） Neural function defect score was significantly lower in edaravone group and the combination group than that in NS group,and in mild hypothermia combined with edaravone group,the neural function defect score was significantly lower than that of edaravone group（P 0.01）.（2） Compared with sham operation group,NS group,SOD activity decreased with increasing MDA content（P 0.01）;and NS compared to the group of edaravone group and combination group,the activity of SOD increased,MDA decreased（P 0.01 ）,and there were significant difference between combined group and edaravone group（P 0.01 ）.（3 ） NS group,edaravone group and mild hypothermia combined with edaravone group pathological examination were different compared with the sham operation group,but mild hypothermia combined with edaravone group were better than edaravone group,and better than that of NS group（P 0.01）.（4） Cerebral ischemia area in NS group,edaravone group and mild hypothermia combined with edaravone group were increased compared with sham operation group,these three decrease and had significant difference（P 0.01）.Conclusion Mild hypothermia combined with edaravone were effective on cerebral ischemia reperfusion injury.