LewisY抗原及黏蛋白1在卵巢上皮性肿瘤中的表达及其相关性

Expression and correlation of Lewis y antigen and mucin 1 in ovarian epithelial tumor

目的探讨LewisY抗原及黏蛋白1（MUCl）在卵巢上皮性肿瘤中的表达及意义，并探讨LewisY抗原与MUCl表达的相关性。方法应用免疫组化sP法检测恶性卵巢上皮性肿瘤60例（其中高分化2l例、中分化21例、低分化18例），交界性卵巢上皮性肿瘤30例，良性卵巢上皮性肿瘤30例及正常卵巢组织20例中Lewisy抗原与MUCl的表达情况，并分析两者之间的关系及其与卵巢癌生物学特性的关系。同时利用免疫荧光双标记法检测Lewisy抗原与MUCl在卵巢癌组织中的结构关系。结果在恶性卵巢上皮性肿瘤中，LewisY抗原的阳性表达率为88．33％，明显高于交界性组（60．00％，X2=9．6405）、良性组（33．33％，X2=28．8095）及正常卵巢组（0，X2=52．3457）（P均〈0．01）；LewisY抗原的阳性表达率与卵巢癌临床病理参数无关，但其表达强度随恶性程度的增加而增加（P〈0．05）。MUCl在卵巢恶性组的阳性表达率为86．67％，明显高于交界性组（53．33％，X2=12．0321）、良性组（30．00％，X2=29．4064）以及正常卵巢组（25．00％，X2=27．8464）（P均〈0．01），其表达强度随临床分期的增加而增加（P〈0．01），与淋巴结转移、组织学分级无关（P均〉0．05）；LewisY抗原和MUCl在卵巢癌组织中同时高表达，其表达具有正相关（r=0．707，P〈0．01），且二者存在共定位关系。结论LewisY抗原及MUCl参与了卵巢癌的发生、发展，可作为判断卵巢癌生物学行为的参考指标，为卵巢癌的生物学治疗提供理论依据。

Objective To investigate the expression and the clinical significance of Lewis y antigen and Mucin 1 （MUC1）, as well as to evaluate the correlation between them in epithelial ovarian tumor. Methods The expression of Lewis y antigen and MUC1 in 60 cases of epithelial ovarian malignant tumors, 30 cases of borderline ovarian tumors, 30 cases of benign ovarian tumors and 20 cases of normal ovarian tissues were detected by immunohistochemical staining. The relationship between Lewis y antigen and MUC1, and their relationship with biology characteristic of ovarian carcinoma were analyzed. An immunofluoreseence double labeling methods was performed to detect the correlation between Lewis y antigen and MUC1. Results In malignant epithelial ovarian tumors, the positive rates of Lewis y antigen was 88. 33 %, which was significantly higher than the positive rates in borderline（60. 00% ,X2 =9. 6405,P 〈0.01） and benign ovarian tumors（33.33% ,X2 =28. 8095,P 〈 O. O1 ） and normal ovarian samples （0, X2 = 52. 3457, P 〈 O. O1 ）. The positive rates of Lewis y antigen had nothing to do with the clinical pathological parameters of ovarian tumor, but the expression intensity of Lewis y antigen was increased with the development of the malignant degree （P 〈 O. 05 ）. The positive rates of MUC1 in malignant epithelial ovarian tumors was also significantly higher than that in borderline, benign ovarian tumors and normal ovarian samples （ 86. 67% vs 53.33%, 30.00%, 25.00% , X2 = 12. 0321,29. 4064,27. 8464 ; P 〈 0. 01 ）. And the expression intensity of MUC1 also increased with the development of clinical stage（ P 〈0. 01 ）, but had nothing to do with the lymph node metastasis and histological grade（P 〉 0. 05 ）. In ovarian cancer, both Lewis y antigen and MUC1 were highly expressed, and their expression levels were positively correlated （r = 0. 707 ,P 〈0.01 ） ,and Lewis y antigen colocalized with MUC1. Conclusion Both Lewis y antigen and MUC1 are associated with the occurrence and development of ovarian cancer. Lewis y antigen and MUC1 might be a sighof biological behavior in ovarian cancers, and this study provides theoretical evidence of ovarian cancer biological treatment.