膀胱癌组织中去整合素金属蛋白酶12和增殖细胞核抗原过度表达的相关性研究

Expression and clinical significance of a disintegrin and metalloproteinase-12 and proliferating cellnuclear antigen in bladder carcinoma

目的探讨去整合素金属蛋白酶12（ADAM-12）和增殖细胞核抗原（PCNA）在膀胱癌中的表达与膀胱癌病理学特点。方法应用免疫组织化学法检测43例膀胱癌、12例正常膀胱组织中ADAM．12及PCNA的表达情况，并分析其与膀胱癌患者的临床特征及病理分期的关系。结果免疫染色标本中，ADAM-12在膀胱癌中的表达明显高于正常膀胱组织，两者比较差异有统计学意义（Z=4．879，P〈0．05）。组织学分化低的表达明显高于中、高分化者（X2=22．3685，P〈0．01）。PCNA在膀胱癌中的表达明显高于正常膀胱组织，两者比较差异有统计学意义（Z=4．879，P〈0．05）。组织学分化低的表达明显高于中高分化者（）（z=10．665，P＝0．0137）。ADAM－12与PCNA在膀胱癌中的表达呈正相关（r=1．000，P〈0．0001）。结论膀胱癌组织中PCNA和ADAM-12蛋白呈过度表达，可能与肿瘤的发生、发展密切相关；ADAM．12是膀胱癌具有潜在价值的肿瘤标记物，可以成为新的一项临床观察指标，因此具有临床应用价值。

Objective To investigate the expression of a disintegrin and metalloproteinase-12 （ADAM12） and proliferating cell nuclear antigen（PCNA） in human bladder carcinoma, and to explore their correlation with different grades and stages of bladder cancer. Methods Biopsies of 12 normal bladder and 43 bladder tumors were performed. And immunohistochemistry was conducted to detect the expression of ADAM12 and PCNA in the biopsies. Results Positive expression signals of ADAM12 were detected significantly higher in the bladder cancer biopsies than that in the normal ones （ Z = 4. 879, P 〈 0. 05 ） , and the expression level of ADAM-12 in lower histological grade was significantly higher than that in the moderate and higher histological grades （ X2 = 22. 3685,P 〈 0. 01 ）. Positive expression signals of PCNA were detected significantly higher in the bladder cancer biopsies than that in the normal ones （ Z = 4. 879, P 〈 0.05 ） ）. Those with lower histological grade had a higher expression level of PCNA when compared with the moderate and higher histological grades （ X2 = 10. 665, P = 0.0137）. The expression of ADAM-12 was positively correlated with PC NA in bladder cancer （r = 1. 000,P 〈0. 0001 ）. Conclusion The over expression of ADAM12 and PCNA maybe play an important role in development of the bladder tumors. And ADAM12 may be a promising biomarker of bladder cancer in the clinical behavior.