小麦TaLTR基因的克隆及初步表达分析

Cloning and Preliminary Expression Analysis of TaLTR Gene from Wheat

以小麦山融3号为试验材料,克隆了TaLTR cDNA序列(Low temperature-responsive RNA-bind-ing protein)。序列分析表明,TaLTR序列包含完整的ORF区,编码蛋白含有162个氨基酸,其氨基端包含一个RRM(RNA recognition domain)超家族保守的结构域,羧基端则富含甘氨酸;经半定量RT-PCR分析,表明TaLTR参与低温、干旱、盐胁迫逆境反应。

The cDNA of a low temperature - responsive RNA - binding protein gene, was isolated from the leaves of wheat variety Shanrong 3 by RT - PCR. The results of named as TaLTR, sequence analysis showed that the TaLTR cDNA had a complete open reading frame （ORF） of 486 bp encoding a protein with 162 amino acids; there was a RNA recognition motif domain （RRM） in amino terminal, and the C -terminus enriched glycine. Through the semi -quantitative RT- PCR, it was found that TaLTR participated in the re- sponse to low temperature, drought and saline stress.