摘要
利用染色体步移技术,从花生品种鲁花14中克隆到716 bp的AhFAD2A基因启动子片段。序列分析表明,该启动子中含有多种种子特异性表达的元件,如GCN4基序、AGCCCA序列元件、Skn-1基序等,可能与AhFAD2A基因在种子中有较高的表达水平有关。启动子中预测的调控元件的功能还需进一步实验验证。
Using the method of chromosome walking, a 716 bp fragment of AhFAD2A gene was cloned from peanut variety Luhua 14. The results of sequence analysis showed that the promoter fragment contained many seed- specific elements or motifs, such as AGCCCA, Skn -1T, GCN4, etc. It might be related with the higher expression of AhFAD2A gene in seed . The function of regulation elements presumed in the promoter need to be verified by further experiments.
出处
《山东农业科学》
2013年第1期30-33,共4页
Shandong Agricultural Sciences
基金
国家自然科学基金项目(31271312)
山东省科技发展计划项目(2012GNC1101)资助
