RAV基因对拟南芥和大豆不定芽再生的影响

Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean

RAV基因是具有AP2/ERF结构域的转录因子家族成员之一,以拟南芥rav突变体和GmRAV干涉(GmRAV-i)大豆的不同外植体为材料进行愈伤组织诱导,分别在芽诱导培养基中加入不同浓度的外源细胞分裂素2-ip和6-BA,考察平均不定芽数和不定芽再生频率。结果表明:与野生型材料相比,拟南芥rav突变体和GmRAV-i大豆在适宜的2-ip和6-BA浓度下平均不定芽数和不定芽再生频率均有所降低。证明RAV基因同系物在不同植物间具有功能保守性,并且在细胞分裂素存在下促进植物不定芽的再生。

RAV is a member of the ERF/AP2 transcription factor family. We use explants of Arabidopsis rav T-DNA insertion homozygous mutants and inhibition（-i） of GmRAV in soybean as experimental material to carry out callus induction. Various concentration of exogenous Cytokinins 2-ip and 6-BA were added in the shoot-inducing medium and the changes in the average number of shoots and the shoot regeneration rates were investigated. It turned out that Cytokinins had positive effects on the shoot regeneration,in addition, the average number of shoots and shoot regeneration rates of Arabidopsis rav mutants and Gm- RAV-i soybean plants would decrease by 2-ip and 6-BA. It proved that the function of RAV homologs was conserved and RAV promoted shoot regeneration in the presence of Cytokinins.