大豆“冀黄13”突变体筛选及突变体库的建立

Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13"

利用甲基磺酸乙酯(ethyl methane sulfonate,EMS)、叠氮化钠(sodium azide,NaN3)和N离子束分别诱变处理大豆品种"冀黄13"的种子。经M2选择,M3、M4代验证,共筛选出茎器官突变体15份,叶器官突变体19份,花器官突变体31份,种子器官突变体3份以及成熟期突变体18份,其中有9份突变体属于复合性状的突变,最终获得77份稳定遗传的突变体。通过F2群体的表型分析,确定1份雄性不育突变体和1份黑种皮色突变体的表型均由单隐性基因控制。本研究所获得的突变体和所构建突变体库将为大豆功能基因组学研究和遗传改良奠定基础。

Mutants and mutant population are the foundation for gene function analysis and crop genetic improvement. Seed of soybean cultivar “Jihuang 13” were treated with ethyl methane sulfonate（ EMS）, sodium azide （NaN3 ）and nitrogen ion beam implantation respectively for screening mutants and constructingmutant population. Fifteen stem related mutants, 19 leaf related mutants ,31 floral related mutants ,3 seed related mutants, and 18 maturity related mutants were obtained after selected in M2 generation, and validated in M3 and M4 generation. Because there were 9 mutants possessing composite pbenotypes ,77 geneti- cally stable mutants were finally screened out. Further genetic analyses for a male sterile mutant and a black seed color mutant were made in F2 segregating population respectively. The results showed that the phenotypes of these two mutants were both controlled by a single recessive gene. The mutants and mutant population obtained would lay a foundation for functional genom- ics analyses and genetic improvement of soybean.