转GsGST14耐盐碱基因大豆的农艺性状调查

Investigation and Analysis of the Main Agronomic Traits of Different Transgenic Soybean Lines with GsGST14 Gene

对前期获得的转耐盐碱基因GsGST14的大豆株系HF55-GST14-1、HF55-GST14-2、HF55-GST14-3、HF55-GST14-4、HF55-GST14-5的T3代群体进行抽样PCR阳性检测,结果后代群体中转基因阳性个体比例高于80%,说明对这些后代群体的调查能够反映出GsGST14基因对转基因大豆农艺性状的影响。因此对这5个株系的T3代群体农艺性状进行调查及统计学分析。结果表明,5个转基因株系与对照在单株荚数、单株粒数、百粒重、生育期、结荚习性、花色、叶形和蛋白质含量上无显著差异,转基因株系HF55-GST14-1、HF55-GST14-3与HF55-GST14-4的株高显著低于对照。油分含量HF55-GST14-1显著高于对照,HF55-GST14-2显著低于对照。因此,5个转GsGST14基因大豆株系并未在农艺性状上产生较大的不良变异,具有良好的应用前景。

In the previous study, we have transformed salt tolerance gene GsGST14 into soybean. In the present study, the ag- ronomic traits,including the yield, morphological and quality traits of five trausgenic soybean lines, as well as the CK variety Hefeng55,were investigated and analyzed statistically. There were no obvious differences between transgeuic soybean and the control in pods per plant, seeds per plant, 100-seed weight, growth duration, podding habits, flower color, leaf shape and protein content,but the plant height of HF55-GST14-1, HF55-GST14-3 and HF55-GST14-4 were obviously shorter than control, and the oil content of HF55-GST14-1 and HF55-GST14-2 were significantly higher and lower than that of the receptor variety, re- spectively. All the results indicated that five transgenic soybean lines with GsGSTI4 gene had no seriously adverse variation in yield, morphological and quality traits, so they would have good utilization prospects.