749例临产妇宫颈分泌物培养鉴定和药敏耐药性分析

Identification of cervical secretion culture and drug-resistance analysis in 749 parturient women

目的通过749例临产妇宫颈分泌物培养鉴定和药敏结果分析,进一步了解病原菌的菌群分布及耐药性,为临床合理使用抗生素提供参考依据。方法对该院2009年1月—2011年12月在产科病房住院的临产妇的749例宫颈分泌物标本通过全自动细菌鉴定仪进行细菌鉴定和药敏试验,并做进一步的回顾性分析。结果从749例临产妇的宫颈分泌物中分离出455例阳性标本,阳性检出率为60.7%;其中革兰阳性菌占45.7%,以凝固酶阴性葡萄球菌为主占31.4%;革兰阴性杆菌占12.6%,以大肠埃希菌为主占7.6%;真菌占2.5%,以白色假丝酵母菌为主。革兰阳性菌对万古霉素、呋喃妥因敏感性最高;革兰阴性杆菌对亚胺培南、哌拉西林/他唑巴坦敏感性最高。结论宫颈分泌物中病原菌菌群分布比较广,为了更好的为患者进行预防和治疗,临床医生应该根据细菌培养及药敏结果结合临床合理应用抗菌药物。

Objective To investigate the distribution of pathogens and drug resistance by analyzing cervical secretion culture and drug sensitivity in 749 parturient women, therefore providing references for reasonable application of antibiotics. Methods Bacterial culture and drug sensitivity testing using automatic bacteria identification system in cervical secretion samples were retrospectively analyzed in 749 parturient women from January 2009 to December 2011. Results Four hundred and fifty-five positive samples were separated from cervical secretion in 749 parturient women with positive rate of 60.7%. Of positive samples, Gram-positive bacteria were 45.7% ,coagu- lase-negative staphylococci 31.4% ,Gram-negative Bacilli 12.6% ,enterohemorrhagic Escherichia coli 7.6% ,fungus 2.5% with promi- nent candida albicans. Gram-positive bacteria were most sensitive to vancomycin and furadantin. Gram-negative Bacilli were most sensi- tive to imipenem, and piperacillin-tazobactam. Conclusions The pathogens have wide distribution in cervical secretion. In order to pro- vide better prevention and treatment for patients, clinical doctors should appropriately use antibiotics according to bacterial culture and drug sensitivity testing.