常州市1～6岁健康儿童EV71血清流行病学研究

Seroepidemiological Study of Enterovirus 71 Infection in Children Aged 1 Through 6 Years in Changzhou in 2006

目的了解手足口病大规模流行之前儿童的肠道病毒71型(EV71)感染率和抗体水平。方法以常州市2006年采集的252名1～5岁健康儿童血清为研究对象,采用中和试验测定血清的EV71中和抗体,以中和抗体阳性作为既往感染标志物。结果 1～5岁儿童EV71平均感染率为32.9%,1～3岁组感染率为9.8%～22.4%,4岁组和5岁组的感染率为43.5%和79.5%,分别与1～3岁组感染率(14.38%)进行比较,差异均有统计学意义。中和抗体几何平均滴度(GMT)在1～3岁组为4.9～8.8,4岁组和5岁组分别为21.8和147.5,组间差异有统计学意义。结论早在2008年全国大规模流行之前EV71在该地区已有一定程度的传播。

Objective To investigate the infection rate and neutralization antibody level of enterovirus 71 （EV71） in healthy children before the national outbreak of hand, foot and mouth disease. Methods Serum samples were collected from 252 healthy children aged 1 - 5 years in Changzhou in 2006. Neutralizing antibody assay was used to detect the serum antibody. The positive neutralization antibody was set as the marker of past infection. Results The average EV71 infection rate in healthy children aged 1 - 5 years was 32.9%, with the relative lower rates of 9.8%, 10.7%, 22.4% in the age groups 1 year, 2 years, and 3 years and the significantly higher rates of 43.5 % and 79.5 % in the age groups 4 years and 5 years, respectively. The infection rate of the age group 1 - 3 years （14.38 % ） was lower than those of the age groups 4 years and 5 years, and the differences were statistically significant. The neutralization antibody geometric mean titers of the age groups 1 year, 2 years, 3 years, 4 years and 5 years were 6.4, 4.9, 8.8, 21.8, and 147.5, respectively. And there were statistically significant differences among the age groups. Conclusions EV71 had spread among the children in Changzhou areas before the national wild outbreak.