护心康对动脉粥样硬化兔TLR4的干预作用

Studies on the Effects of Huxinkang on TLR4 in Atherosclerosis Rabbits

目的研究护心康对兔动脉粥样硬化防治的作用机制。方法采用高脂喂饲复制兔动脉粥样硬化模型,将护心康分为低、中、高剂量3个不同剂量组进行干预治疗,检测给药后动脉管壁TLR4中的变化。结果护心康中、高剂量均有较好的降低兔动脉管壁TLR4的作用,高剂量作用优于低剂量,差异有统计学意义(P<0.05)。结论护心康具有降低动脉管壁TLR4的功效。

Objective To explore the mechanism of atherosclerosis （AS） prevention and treatment by Huxinkang in rabbits. Methods 90 male Chinese white rabbits were randomly divided into 5 groups： normal group, model group, Huxinkang low, middle and high dose group. The rabbits in all the groups except for the normal group were fed with high cholesterol diet for 63 days. From the 30th day after the finish of the modeling, the rabbits in the Huxinkang low, middle and high dose group were respectively orally administered with Huxinkang at dcse of 0.27, 0.54 and 1.08 g/kg. d for 4 weeks, while the normal group and model group were administered with distilled water. Then the aorta was collected for toll- like receptors 4（TLR4）detection in the AS plaque. Results Compared with the normal group, the expression of TLR4 in the AS plaque was markedly increased in the other groups（ P 〈 0.05 ）. Huxinkang at dc^es of 0.54 and 1.08 g/kg. d could significantly decrease the level of TLR4（ P 〈 0.05）, and the 1.08 g/kg. d dose was superior to the 0.27 g/kg. d dose（P〈0.05）. There was not statistical difference between the 0.54 g/kg. d and 1.08 g/kg. d dose group （P〈0.05）. Conclusions Huxinkang can decrease the expression of TLR4 in the atherosclerosis plaque in the atherosclerosis rabbits.