巢式PCR在输入性疟疾诊断和分型中的应用价值

Application of Nested PCR in the Diagnosis and Typing of Imported Malaria

目的评价巢式PCR方法用于输入性疟疾病例检测的应用价值。方法引用Snounou and Perandin F等人描述的引物,对本实验室所冻存标本进行巢式PCR检测,并与厚薄血膜显微镜镜检结果进行比较。结果两种方法结果差异有统计学意义,似然比χ2=21.801,P=0.010,且在分型上较厚薄血膜显微镜镜检方法容易被操作者接受。结论在当前应对输入性疟疾的特殊时期,采用巢式PCR对输入性疟疾进行实验室检测和分型,不仅可以作为显微镜检查的有效补充,并且能够发挥其灵敏度和特异性高的优势。

Objective To evaluate the application value of nested PCR in the diagnosis and typing of imported malaria. Methods The frozen whole hlcod samples were tested for malaria by nested PCR. The test results were compared with those by microscopic examination of thick and thin blood films. Results The test results of the two detection methods were significantly different （for the likelihood ratio, ：2 = 21. 801, P = 0. 010）. Typing by nested PCR was more easily accepted by the operators. Conclusions During the current special period of coping with the imported malaria, detection and typing of imported malaria by nested PCR can be used as an important supplement of microscopic examination. It has high sensitivity and specificity.