超高压辅助离子液体提取/HPLC分析牛蒡子中牛蒡苷与牛蒡苷元

Extraction of Arctiin and Arctigenin in Fructus Arctii by Ionic Liquid-based Ultrahigh Pressure Extraction and HPLC

通过离子液体类型与浓度、提取压力、时间、料液比等单因素实验确定了超高压辅助离子液体提取牛蒡子中牛蒡苷和牛蒡苷元的最佳工艺。结果表明,超高压离子液体提取最佳工艺为以0.80 mol/L溴化1-十二烷基-3-甲基咪唑溶液为溶剂,提取压力200 MPa,提取时间2 min,料液比1∶20(g/mL),牛蒡苷和牛蒡苷元的提取率分别为37.15、8.04 mg/g。与传统提取方法相比,超高压提取时间只需2 min,是超声方法的1/15、加热回流方法的1/60,极大地节省了提取时间,提高了工作效率,且不污染环境,是提取牛蒡苷和牛蒡苷元的一种简单有效的方法。

Ionic liquid-based ultrahigh pressure-assisted extraction （ IL - UPE） combined with high performance liquid chromatography（HPLC） was applied in extracting arctiin and arctigenin in Fructus Arctii. The influences of experimental factors including ionic liquids with different cations and anions, ionic liquid concentration, extraction pressure, extraction time and ratio of solid to liquid, were in- vestigated. The optimal conditions were as follows： extraction solvent： 0. 80 mol/L 1-1auryl-3-meth- ylimidazolium bromide ionic＇liquid solution, extraction pressure ： 200 MPa, extraction time ： 2 min, solid/liquid ratio： 1 ： 20（g/mL）. Under the optimum conditions, the yields of arctiin and arctige- nin are 37. 15 mg/g and 8. 04 mg/g, respectively. Compared with the conventional extraction meth- ods （ e. g. the ultrasonic assisted extraction （UAE） and the heat reflux extraction （ HRE ） ）, the IL - UPE method was rapid（extraction time： 2 min）, effective and also environment-friendly.