高效液相色谱法测定不同单一食物下短翅豆芫菁体内斑蝥素的含量

Quantitative determination of cantharidin in Epicauta aptera Kaszab with different food by HPLC

运用高效液相色谱法测定不同单一食物下短翅豆芫菁体内斑蝥素的含量。采用HPLC法,色谱柱为C18柱,流动相为甲醇∶水(28∶72),检测波长为230nm,进样量为20μL,柱温为30℃。用丙酮氯仿在酸性条件下回流提取虫粉。处理的6个样品中,在西红柿叶喂养条件下短翅豆芫菁体内斑蝥素的含量高于马兰叶。

Quantitative determination of cantharidin in Epicauta aptera Kaszab with different food by HPLC. Waters 2487 HPLC and C18 column was used to set the mobile phase at methanol-water（28： 72）, the column temperature is 30℃, the flow rate is lmL/min, the detection wavelength is 230nm, With acetone and chloroform in acid conditions backflow is to extract insect powder. The cantharidin content in the tomato leaves feeding conditions is higher than Kalimeris indica leaves in dealing with six samples.