三种因素对痰培养结果的影响

Influence of the three factors on sputum culture results

目的通过分析痰标本阳性级别、患者治疗情况和储存时间等三种因素对培养结果的影响,为基层实验室开展临床痰培养提供参考依据。方法对江苏连云港第四人民医院实验室2010年1～10月痰培养数据进行分析。结果该实验室涂阳患者痰培养共计611份,分别统计了痰标本阳性级别、患者治疗情况和储存时间对培养结果的影响,结果表明标本的储存时间对涂阳培阴率和污染率都有显著影响,痰标本的阳性级别和患者的治疗情况仅影响涂阳培阴率,而不影响污染率。结论基层实验室在开展痰培养临床诊断时应提高标本质量,并且在收集到标本3d内完成痰培养;此外,需要进一步摸索适用于低阳性级别和复治患者的痰培养的方法,以保证痰菌培养获得理想的结果。

Objective To analyze the effect of positive grade of sputum,treatment status and storage time of sputum to the outcome of sputum culture,and to provide reference for clinical sputum culturing.Methods The sputum culture data from the Fourth People＇s Hospital of Lianyungang City between January and October in 2011 were analyzed.Results The influences of positive grade of sputum,treatment status and storage time of sputum towards culture result were analyzed among 611 sputum samples cultured from the laboratory.The results revealed that the storage time of sputum influenced the smear positive-culture negative rate and contamination rate significantly.In addition,the positive grade of sputum,treatment of patients only played a significant effect on smear positive-culture negative rate rather than contamination rate.Conclusion The basic laboratories should improve the quality of sputum samples when carrying out clinical diagnosis,and the culture process is required to be completed in 3 days after collection of sputum.In addition,it was necessary to improve the culture method for sputum of low positive grade and from re-treated patients in order to obtain ideal culture results.