毒鼠强对大鼠脑细胞色素C表达的影响

Expression of Cyt C in Brain of Tetramine Toxic Rats

目的:探讨毒鼠强慢性中毒的大鼠脑部细胞色素C的表达情况。方法:建立SD大鼠毒鼠强慢性中毒模型,检测中毒后不同时间大鼠脑部细胞色素C的表达。结果:对照组大鼠脑部组织细胞色素C仅有微量表达,实验组大鼠中毒后30 min脑部组织细胞色素C的表达开始增强,1～3 d达高峰,4～5 d后开始下降,10 d后基本恢复到正常水平。结论:大鼠脑部组织细胞色素C随时间的变化可为推断毒鼠强慢性中毒的不同时期提供法医学证据。

Objective： To explore the evidence for the judgement on legal cases of intoxation by tetramine. Method ：60 rats were given tetramine by lavage and were sacrificed at different time after intoxation. The expression of Cyt C was detected by immunohistochemistry, and the reasults were assessed by an image analysis system. Results：There was a slight expression of Cyt C in control group. In intoxation group the expression of Cyt C increased when the rats were given tetramine after 30 minutes;it reached the peak at 24 hours after intoxation and kept up the level for 3 days, then it began to decrease and returned to the control level in 1 0 days. Conclusion：The regular change of Cyt C expression might be a useful sign for assessment in legal cases of tetramine intoxation.