GC-MS分离鉴定黄芪、白芍及药对中的弱极性成分

Separation and Identification of Weak Polar Components from Astragali Radix,Paeoniae Radix Alba and Herb pairs by GC-MS

目的建立用气相色谱-质谱(GC-MS)法快速检测黄芪、白芍及药对中弱极性成分的方法。方法石油醚提取黄芪、白芍及药对中弱极性成分,并用GC-MS法鉴定。结果从黄芪、白芍单药及药对中共鉴定出15种成分,其中大多数为高级脂肪酸及其酯化物,以亚油酸、亚油酸甲酯含量最为丰富。其中,丹皮酚、丁香酚等物质具有抗氧化效果。此外,在药对中还鉴定出单药中未出现的丁子香烯、β-谷甾醇、羽扇烯酮等。结论本方法快速、简便,可用于分析鉴定中药中弱极性成分。

Objective To establish a GC-MS method for rapid detection of weak polar components from Astragali Radix, Paeoniae Radix Alba and herb pairs. Methods The weak polar components were extracted with petroleum ether and were identified by GC-MS. Results Fifteen compounds were identified, and most of these compounds were higher fatty acids, especially linoleic acid and linoleic acid methyl ester. Paeonol and Eugenol were found to have an antioxidant activity. In herb pairs, there were compounds such as caryophyllene, -sitosterol and lupenone which were not found in single drug. Conclusion This can be a quick and simple method to analyze weak polar components of herbs.