金银花主要成分对铜绿假单胞菌多耐药菌株的抑制作用研究

Study on Inhibitory Effect of Main Antimicrobial Components from Honeysuckle against Resistant Pseudomonas Aeruginosa

目的研究金银花主要成分对铜绿假单胞菌质控菌株和多耐药菌株的体外抑菌作用,及金银花有机酸总样与氨基糖苷类抗生素联合使用对多耐药菌株的抑菌效果。方法平板倍比稀释法测定金银花中主要成分绿原酸、异绿原酸组分和木犀草素以及氨基糖苷类抗生素硫酸阿米卡星和庆大霉素对铜绿假单胞菌质控菌株和耐药菌株的最低抑菌浓度(MIC),在此基础上研究金银花有机酸总样与硫酸阿米卡星联合用药的抑菌效果。结果金银花主要成分绿原酸、异绿原酸组分和木犀草素对铜绿假单胞菌多耐药菌株的MIC分别为10,5和2.5 mg/mL,对质控菌株的MIC分别为>10,5和2.5 mg/mL。金银花有机酸总样与氨基糖苷类抗生素联合用药,可降低硫酸阿米卡星对多耐药铜绿假单胞菌的MIC,两者联用有一定的协同作用。结论金银花主要成分绿原酸、异绿原酸和木犀草素均有抑制铜绿假单胞菌的作用,其中绿原酸对耐药菌株的抑制作用最强。金银花有机酸组分与氨基糖苷类抗生素联合用药,对铜绿假单胞菌多耐药菌株具有协同抑制作用。

Objective To study the antimicrobial activities in vitro of honeysuckle main components against the standard and resistant Pseudomonas aeruginosa and the effect of organic acid components of honeysuckle combined with aminoglycosides on resistant Pseudomonas aeruginosa. Methods The minimum inhibitory concentrations （MICs） of chlorogenic acid, isochlorogenic acids, luteolin and gentamycin and amikacin of aminoglycosides were determined by the constant dilution method against the standard and resistant Pseudomonas aeruginosa. Furthermore, the effect of organic acid components in combination with amikacin on resistant Pseudomonas aeruginosa was tested. Results The MICs of chlorogenic acid, isochlorogenic acids and luteolin were respectively 10 mg/mL, 5 mg/mL and 2.5 mg/mL against the resistant Pseudomonas aeruginosa and were respectively 〉 10 mg/mL, 5 mg/mL and 2.5 mg/mL againstthe standard Pseudomonas aeruginosa. Chlorogenic acid had much stronger inhibition to the resistant Pseudomonas aeruginosa compared to standard strain. The MIC of amikacin against the resistant Pseudomonas aeruginosa was decreased when combined with the organic acid components of honeysuckle for synergy effects. Conclusion Chlorogenic acid, isochlorogenic acids and luteolin, the main active components of honeysuckle, have inhibition effect against both standard and resistant Pseudomonas aeruginosa strains, especially inhibition effect of chlorogenic acid against resistant Pseudomonas aeruginosa strain. The organic acid components of honeysuckle and amikacin show synergy effects of antimicrobial activity to inhibit the resistant Pseudomonas aeruginosa strain.