HPLC测定过山厥提取物中山柰素含量

Determination of Kaempferol in Camptosorus sibiricus by HPLC

目的建立高效液相色谱法测定过山厥提取物中山柰素含量。方法 Hypersil ODS2(4.6 mm×200 mm,5μm)色谱柱,甲醇-0.07%磷酸(50:50)为流动相,检测波长268 nm,流速1.0 mL/min,柱温25℃。结果山柰素浓度在4.99～12.98mg/L线性关系良好,回归方程为Y=58 735X-44 779(R2=0.9983);平均回收率99.8%,RSD=0.97%;重复性良好,RSD=1.14%(n=5)。结论该方法简单、可靠、专属性强,可用于过山厥提取物中山柰素的含量测定。

Objective To establish an HPLC method for the determination of kaempferol in Camptosorus sibiricus. Method Hypersil ODS2 column （4.6 mm×200 mm, 5 μm） was adopted, methanol-0.07% phosphoric acid solution （50：50） as mobile phase, the flow rate wasl.0 mL/min, the detection wavelength was 268 nm and column temperature was room temperature. Results Kaempferol showed a good linear relationship with a range of 4.99- 12.98 mg/L and regression equation was Y=58735X-44779 （R2=0.9983） . The average recovery was 99.8%, RSD= 0.97%, with good repeatability （RSD=1.14%, n=5）. Conclusion The HPLC method was simple, accurate and reproducible, and can be used for the determination ofkaempferol in Camptosorus sibiricus.