摘要
目的建立HPLC测定托吡卡胺滴眼液中苯扎溴铵含量的方法。方法以氰基键合硅胶为填充剂,流动相乙腈-水-三乙胺(440:560:1),磷酸调pH 5.0,检测波长262 nm,流速1.0 mL/min。结果苯扎溴铵在15～45μg/mL范围内呈良好的线性关系(r=0.9993),平均回收率98.8%(n=9),RSD=0.9%。结论 HPLC方法简便灵敏准确,重复性好,可用于苯扎溴铵的质量控制。
Objective To determine the content of benzalkonium bromide in Tropicamide Eye Drops by HPLC. Methods A CN column was used with the mobile phase of water-acetonitrile-triethylamine（440：560：1）. The phosphoric acid was used to adjust pH to 5.0. The detection wavelength was 262 nm. The flow rate was 1.0 mL/min. Results The calibration curve was linear in the range of 15-45 μg/mL for benzalkonium bromide （r = 0.999 3 ） . The average recovery was 98.8 % （n=9, RSD=-0.9 %） for benzalkonium bromide. Conclusion This method is simple, sensitive and accurate with a good repeatability for the quality control of Tropicamide Eye Drops.
出处
《食品与药品》
CAS
2013年第1期47-49,共3页
Food and Drug
