硬化性肌膜炎患者血清ACA的发现及对小鼠细胞着丝点的研究

DISCOVERY OF THE ANTIBODY TO KINETOCHORE FROM SERUM OF PATIENTS WITH SCLEROTIC FASCIITIS AND THE RESEARCH ON KINETOCHORE OF SEVERAL ORGANS IN MICE WITH THIS ANTIBODY

首次在硬化性肌膜炎患者血清中发现有抗着丝点抗体(ACA),可使 Hep-2间期细胞核及 M 期细胞染色体着丝点显示分散的荧光斑点.用 ACA 血清对小鼠几种器官的冰冻切片或涂片进行免疫荧光研究,可见无论幼龄或老龄小鼠的肝、十二指肠、脑皮层细胞及老龄小鼠睾丸生精细胞均有明亮的荧光斑点.绒毛顶端细胞呈均匀性荧光.

The antibody to kinetochore is discovered in the serum of patients with solerotic fasciitis for the first time,since the discrete fluores- cence speckles are visualized on the nuclei in inter-phase and chromosomes in M-phase of Hep-2 cells by inderict immuno-fluorescence.In the frozen and smear sections,discrete fluorescence speckles are visualized on the nuclei of all cells of spermatogenesis (old mice),crypts and villi of duodenum, cortex and liver in old and young mice,but on the nucclei of the epithelium cells of villi at the top in young,particularly in old mice,the homogeneous fluorescence in nuclei appeared.