骆驼蓬碱和去氢骆驼蓬碱的质谱裂解途径研究

The ESI /MS Study on the Fragmentation Pathways of the Harmaline and Harmine

目的:研究去氢骆驼蓬碱和骆驼蓬碱的质谱裂解机制。方法:两者用HPLC进行分离后,在正离子模式下,得到去氢骆驼蓬碱和骆驼蓬碱的质谱图。结果:解析了去氢骆驼蓬碱和骆驼蓬碱多级质谱碎片,主要发生RDA裂解、-CH3和-OH等自由基丢失,吲哚环稳定。结论:本研究丰富了去氢骆驼蓬碱和骆驼蓬碱的质谱裂解机制,为β-咔啉类生物碱的成分研究提供依据。

Objective ：To study the fragmentation pathways of harmaline and harmine by etectrospray ionization mass spectrometry （ ESI- MSn）. Methods：Harmaline and harmine were analyzed by ESI-MSn in positive ion mode. The first and multi-stage mass spectrum dia- grams were obtained. Results： To analyze mass spectrometry fragments of the harmaline and harmine, under the positive mode, RDA decomposition occurred mainly, and - CH3, -OH were lost. Conclusion： The results enriched the mass spectrum decomposition, and provided the basis for the chemical constituents research of B-carboline alkaloid.