大孔吸附树脂纯化板栗壳总黄酮的研究

Purification Technology of Total Flavonoids in Chestnut Shell with Macroporous Resin

[目的]为板栗壳总黄酮的纯化筛选合适的工艺。[方法]采取静态和动态吸附/解吸附结合的方法,以解吸附率和黄酮含量为主要指标对板栗壳总黄酮的纯化进行研究。[结果]4种大孔吸附树脂中,AB-8最适合纯化板栗壳总黄酮,纯化工艺为:按9.1 mg黄酮/g树脂,以2 BV/h的速度上样,用45%乙醇,2 BV/h的速度洗脱,所得总黄酮含量达47.86%。[结论]大孔吸附树脂AB-8对板栗壳总黄酮具有较好的纯化效果。

[Objective] To establish the optimum purification conditions of total flavonoids in chestnut shell with macroporous resin.[Method] With desorption rate and flavonoid content as main indicator,the technological conditions of purification were examined by the static adsorption and desorption experiment as well as dynamic adsorption and desorption tests.[Result] Among the four kinds of macroporous resin,AB-8 was best for the purification of total flavonoids from chestnut shell.The flavonoid content could amount to 47.86% with the way which the extract were loaded at the rate of 2 BV/h,according 9.1 mg flavonoid/g resin,and eluted at 2 BV/h.[Conclusion] The total flavonoids in chestnut shell could be well purified with AB-8.