TMEM16A在结肠癌组织中的表达及其意义

Significance of TMEM16A expression in colorectal carcinoma

目的:探讨Ca2+激活的氯离子通道(Ca2+-activated Cl-channels,CaCC)蛋白TMEM16A在结肠癌中的表达和意义.方法:收集67例结肠癌标本,采用免疫组织化学SP法检测TMEM16A的表达,以癌旁结肠黏膜组织和6例结肠腺瘤组织作为对照,1例胃间质瘤组织作为阳性对照.结果:TMEM16A表达于结肠癌细胞胞膜和胞质内,在67例结肠癌中TMEM16A呈不同程度阳性表达,其中阴性占5.97%(4/67)、弱阳性占16.42%(11/67)、阳性占29.85%(20/67)、强阳性占47.76%(32/67);在癌旁结肠黏膜腺体内大多呈阴性和弱阳性表达,其中阴性占40.30%(27/67)、弱阳性占52.24%(35/67)、阳性占4.48%(3/67)、强阳性占2.99%(2/67);在6例结肠腺瘤中均呈阳性表达.将阴性和弱阳性表达分为阴性组,将阳性和强阳性表达分为阳性组进行分析,在67例结肠癌中TMEM16A表达的总阳性率("阳性"和"强阳性")占77.61%(52/67),而在相应的癌旁组织中TMEM16A表达的总阳性率("阳性"和"强阳性")仅占7.46%(5/67),两者之间差异显著(P<0.005).结论:TMEM16A可高表达于结肠癌组织,可作为结肠癌的分子诊断和靶向治疗的一个新的候选靶点.

AIM：To investigate the expression of transmembrane protein 16A （TMEM16A） in colorectal carcinoma. METHODS：The expression of TMEM16A was detected by immunohistochemistry in 67 surgical colorectal carcinoma specimens and matched tumor-adjacent colorectal specimens. RESULTS：TMEM16A was expressed in both cytoplasm and cell membrane. Among 67 colorectal carcinoma specimens, TMEM16A expression was negative in 4 cases （5.97%）, weakly positive in 11 cases （16.42%）, positive in 20 cases （29.85%）, and strongly positive in 32 cases （47.76%）. Among 67 tumor-adjacent healthy tissue specimens, TMEM16A expression was negative in 27 cases （40.30%）, weakly positive in 35 cases （52.24%）, positive in 3 cases （4.48%）, and strongly positive in 2 cases （2.99%）. The positive （＂positive＂ plus ＂strongly positive＂） rate of TMEM16A expression was significantly higher in colorectal carcinoma tissue than in tumor adjacent healthy tissue （77.61% vs 7.46%, P 0.005）. CONCLUSION：Aberrant expression of TMEM16A is a frequent feature in colorectal carcinoma. TMEM16A can be used as a new candidate target for diagnosis and treatment of colorectal carcinoma.