摘要
目的:探讨KLF6(krüppel-like factor 6)在胃癌和癌前病变中的表达及其意义.方法:收集郑州大学第二附属医院手术切除32例胃癌组织及其配对的正常胃黏膜组织新鲜标本,采用RT-PCR检测组织中KLF6 mRNA的表达.同期收集20例正常胃黏膜、45例肠上皮化生、16例异型增生及30例胃癌组织存档蜡块,采用免疫组织化学SP法检测组织中KLF6蛋白的表达,并结合临床资料进行分析.结果:正常胃黏膜与Ⅲ型肠上皮化生、异型增生及胃癌组间KLF6蛋白的表达差异有统计学意义(均P<0.05);KLF6蛋白的表达与肿瘤分化程度、肿瘤大小相关,差异有统计学意义(均P<0.05).正常胃黏膜与胃癌组织KLF6 mRNA表达差异无统计学意义(P=0.357).结论:KLF6蛋白的表达异常与Ⅲ型肠化、异型增生、胃癌的发生发展密切相关,其在监测癌前病变的进展及早期胃癌的发现中有一定价值.
AIM:To detect the expression of the krüppel-like factor 6(KLF6) in human gastric carcinoma and premalignant gastric lesions to investigate its role in the occurrence and development of gastric cancer.METHODS:Fresh frozen gastric carcinoma tissues and matched tumor-adjacent normal gastric mucosa tissues were collected from 32 patients with gastric carcinoma.RT-PCR was used to analyze the expression level of KLF6 mRNA in these tissue samples.Meanwhile,the expression of KLF6 protein was detected by immunohistochemistry in 111 formalin-fixed paraffin-embedded samples,including 20 cases of normal gastric mucosa,45 cases of intestinal metaplasia,16 cases of dysplasia,and 30 cases of gastric carcinoma.The association of KLF6 expression with clinicopathological characteristics was analyzed.RESULTS:The positive rate of KLF6 protein expression in normal gastric mucosa was significantly different from those in type Ⅲ intestinal metaplasia,dysplasia and gastric carcinoma(χ2=6.033,3.889,11.092,P=0.014,0.049,0.001).The positive rate of KLF6 expression in gastric carcinoma was associated with tumor differentiation and size(P=0.009,0.010).There was no significant difference in the expression of KLF6 mRNA between normal gastric mucosa and gastric carcinoma(P=0.357).CONCLUSION:Abnormal expression of KLF6 is closely related to type Ⅲ intestinal metaplasia,dysplasia,and gastric carcinoma.Detection of KLF6 expression may have certain value in the surveillance of premalignant lesions and the discovery of early gastric carcinoma.
出处
《世界华人消化杂志》
CAS
北大核心
2012年第36期3683-3688,共6页
World Chinese Journal of Digestology
基金
河南省自然科学研究计划基金资助项目
No.2011A320062
河南省医学科技攻关计划基金资助项目
No.201203047~~
关键词
KLF6
胃癌
异型增生
肠上皮化生
krüppel-like factor 6
Gastric carcinoma
Dysplasia
Intestinal metaplasia