KLF6在胃癌和癌前病变中的表达及其意义

Expression of KLF6 in gastric carcinoma and premalignant gastric lesions

目的:探讨KLF6(krüppel-like factor 6)在胃癌和癌前病变中的表达及其意义.方法:收集郑州大学第二附属医院手术切除32例胃癌组织及其配对的正常胃黏膜组织新鲜标本,采用RT-PCR检测组织中KLF6 mRNA的表达.同期收集20例正常胃黏膜、45例肠上皮化生、16例异型增生及30例胃癌组织存档蜡块,采用免疫组织化学SP法检测组织中KLF6蛋白的表达,并结合临床资料进行分析.结果:正常胃黏膜与Ⅲ型肠上皮化生、异型增生及胃癌组间KLF6蛋白的表达差异有统计学意义(均P<0.05);KLF6蛋白的表达与肿瘤分化程度、肿瘤大小相关,差异有统计学意义(均P<0.05).正常胃黏膜与胃癌组织KLF6 mRNA表达差异无统计学意义(P=0.357).结论:KLF6蛋白的表达异常与Ⅲ型肠化、异型增生、胃癌的发生发展密切相关,其在监测癌前病变的进展及早期胃癌的发现中有一定价值.

AIM：To detect the expression of the krüppel-like factor 6（KLF6） in human gastric carcinoma and premalignant gastric lesions to investigate its role in the occurrence and development of gastric cancer.METHODS：Fresh frozen gastric carcinoma tissues and matched tumor-adjacent normal gastric mucosa tissues were collected from 32 patients with gastric carcinoma.RT-PCR was used to analyze the expression level of KLF6 mRNA in these tissue samples.Meanwhile,the expression of KLF6 protein was detected by immunohistochemistry in 111 formalin-fixed paraffin-embedded samples,including 20 cases of normal gastric mucosa,45 cases of intestinal metaplasia,16 cases of dysplasia,and 30 cases of gastric carcinoma.The association of KLF6 expression with clinicopathological characteristics was analyzed.RESULTS：The positive rate of KLF6 protein expression in normal gastric mucosa was significantly different from those in type Ⅲ intestinal metaplasia,dysplasia and gastric carcinoma（χ2=6.033,3.889,11.092,P=0.014,0.049,0.001）.The positive rate of KLF6 expression in gastric carcinoma was associated with tumor differentiation and size（P=0.009,0.010）.There was no significant difference in the expression of KLF6 mRNA between normal gastric mucosa and gastric carcinoma（P=0.357）.CONCLUSION：Abnormal expression of KLF6 is closely related to type Ⅲ intestinal metaplasia,dysplasia,and gastric carcinoma.Detection of KLF6 expression may have certain value in the surveillance of premalignant lesions and the discovery of early gastric carcinoma.