Caspase-3在慢性病毒性乙、丙型肝炎及非酒精性肝炎中的表达及意义

Significance of expression of caspase-3 in chronic hepatitis B and C and nonalcoholic steatohepatitis

目的:检测慢性乙型肝炎、慢性丙型肝炎和非酒精性脂肪性肝炎中Caspase-3的表达,探讨其临床及病理学意义.方法:应用免疫组织化学方法检测Caspase-3在70例慢性乙型肝炎,50例慢性丙型肝炎,50例非酒精性脂肪性肝炎和15例正常肝组织的表达.结果:(1)Caspase-3在慢性乙型肝炎、慢性丙型肝炎、非酒精性脂肪性肝炎中的表达(57.1%、72.0%、82.0%),高于其在正常肝组织中表达(18.8%),差异有统计学意义(P<0.05);并与肝细胞凋亡密切相关(r=0.356,P<0.001);(2)Caspase-3在慢性乙型肝炎、慢性丙型肝炎和非酒精性脂肪性肝炎随病理损伤程度加重其阳性表达率增高,有统计学意义(P<0.05).结论:Caspase-3与慢性乙型肝炎、丙型肝炎和非酒精性脂肪性肝炎的肝细胞凋亡密切相关,并参与肝炎的发病过程.

AIM：To determine the expression of caspase-3 in liver tissues of patients with chronic hepatitis B（CHB）,chronic hepatitis C（CHC）,or nonalcoholic steatohepatitis（NASH） and to analyze their clinical and pathological significance.METHODS：Immunohistochemistry was used to determine the expression of caspase-3 in liver tissues from 70 patients with CHB,50 patients with CHC,50 patients with NASH,and 15 normal people.RESULTS：The positive rates of caspase-3 expression in CHB,CHC and NASH were significantly higher than that in normal hepatic tissue（57.1%,72.0%,82.0% vs 18.8%;all P〈0.05）.Caspase-3 expression was closely related with hepatic cell apoptosis（r=0.356,P〈0.001）.The positive rates of caspase-3 expression increased with the increase in the degree of pathological injury（all P〈0.05）.CONCLUSION：The expression of caspase-3 in CHB,CHC and NASH is closely related with liver cell apoptosis.Overexpression of caspase-3 may play an important role in the development of hepatitis.