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Btk及NFκB在急性髓系白血病细胞中的表达及其意义 被引量：5

Expression of Btk and NFκB in Acute Myeloid Leukemia Cells and Its Significance
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摘要 本研究检测Btk及NFκB在急性髓系白血病(AML)患者白血病细胞中的表达,评价其在AML发生、发展中的作用。选取14例AML初诊及治疗达完全缓解患者的骨髓单个核细胞标本,检测Btk、NFκB在mRNA及蛋白质水平的表达。结果表明:在mRNA及蛋白质水平,AML患者细胞中均有Btk、NFκB的表达。Btk、NFκB蛋白在初发AML患者白血病细胞中表达高,达完全缓解后,其表达下降或检测不到(P<0.05)。结论:Btk、NFκB在AML的发生、发展中可能起重要作用,它们有可能作为AML的潜在治疗靶点及用于预后预测。 This study was purposed to investigate the expression of Btk and NFκB in acute myeloid leukemia （AML） cells and its significance. Bone marrow mononuclear cell specimens were taken from 14 AML patients who were in new diagnosis and complete remission respectively, the expressions of Btk and NFκB at mRNA and protein levels were detected by RT-PCR and Western blot, respectively. The results showed that Btk and NFκB expressed in all the samples at RNA and protein levels. At protein level, Btk and NFκB expressions were higher in the cells from newly diagnosed AML patients than that in the cells from patients in complete remission stage （ P 〈 0.05 ）. It is concluded that Btk and NFκB may play an important role in the development and progression of AML, they may be used as potential therapeutic targets of AML and used in predicting the prognosis.
作者 陶善东 邓媛 何正梅 陈月 邓之奎 李元媛 朱家斌 丁邦和 于亮
机构地区 南京医科大学附属淮安第一医院血液科
出处 《中国实验血液学杂志》 CAS CSCD 北大核心 2013年第1期25-28,共4页 Journal of Experimental Hematology
基金 国家自然科学基金资助项目(编号81170489) 江苏省自然科学基金资助项目(编号BK2010287) 江苏省卫生厅基金资助项目(编号H201056)
关键词 急性髓系白血病 BTK NFΚB acute myeloid leukemia Btk NFκB
分类号 R733.71 [医药卫生—肿瘤]
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参考文献16

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二级参考文献5

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共引文献4

同被引文献37

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中国实验血液学杂志
2013年 第1期

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