小菜蛾溴氰菊酯敏感和抗性品系蛋白质表达谱的比较分析(英文)

A comparative profiling of protein expression in the deltamethrin-sensitive and resistant strains of the diamondback moth (Plutella xylostella)

杀虫剂抗性是害虫防治中的一个主要挑战。为有效治理抗性,我们必须了解杀虫剂诱导的害虫生理生化变化。目前害虫抗药性的一些机理已经清楚,但更多的相关机制还有待探究。本研究通过蛋白质组学方法检测了小菜蛾Plutella xylostella溴氰菊酯敏感和抗性品系间蛋白质组的表达差异。结果显示:SDS-PAGE胶上有大约300个蛋白差异点,其中23个蛋白点具2.5倍以上的表达差异,通过MALDI-TOF-MS,我们成功鉴定出8个蛋白,其中包括化感蛋白CSP2、铜锌超氧化物歧化酶和peroxiredoxin样蛋白。通过实时定量PCR(real-time quantitativePCR,qPCR)分析了其中5个蛋白的mRNA表达水平,结果表明mRNA表达水平不能真实反映蛋白的表达水平。免疫印迹验证了双向电泳中SOD1的表达差异。本研究有力地证明溴氰菊酯诱导小菜蛾成虫蛋白质组表达变化,这为进一步筛选抗性靶标提供很大的帮助。

A major challenge in pest management is insecticide resistance. To effectively prevent and manage resistance, it is essential to understand the biochemical and physiological changes induced by insecticides. Some molecular mechanisms that are responsible for insecticide resistance were well known whereas others are to be discovered. So the comparative proteomic approach was employed to investigate the proteomie differences between the deltamethrin-sensitive strain （DS） and the deltamethrin-resistant strain （DR） of the diamondback moth （Plutella xylostella L. ）. In this study, about 300 differentially expressed protein spots were detected on each SDS-PAGE gel, among which 23 proteins had a 2.5-fold expression difference between the two strains. Eight proteins were successfully identified by MALDI-TOF- MS, including chemosensory protein CSP2, Cu/Zn superoxide dismutase （SOD1）, peroxiredoxin-like protein, etc. Expression of the five proteins at the mRNA level was analyzed by real-time quantitative PCR, and the results showed that the mRNA expression level did not fully reflect the protein expression level. Western blotting validated the proteomie result of SOD1. This study provides strong evidence that dehamethrin can induce the proteomie change in adult diamondback moth, and is helpful to understanding the resistance development and selecting the action targets of deltamethrin.