人脐带血单核细胞中的神经样细胞研究

Study on neuron-like cells in human umbilical cord blood mononuclear cells

目的研究人脐带血单核细胞(HUBMNCs)中是否存在神经样细胞。方法采集30例新生儿的脐带血,从中分离出单核细胞。采用免疫荧光染色法观察其神经巢蛋白和神经元特异性烯醇化酶(NSE)的表达,并对HUBMNCs进行体外培养,用倒置相差显微镜观察细胞形态。结果 HUBMNCs经免疫荧光染色后,在1个高倍视野(HP,×200)下分别可见(2.67±1.29)个、(7.37±2.46)个散在分布的nestin、NSE阳性的细胞。培养10～12 d时,出现3～5个/HP胞体较大、有数个类似轴突和树突状粗长突起的神经样细胞。结论 HUBMNCs中有少数神经样细胞。

Objective To explore whether neuron-like cells in human umbilical cord blood mononuclear cells （HUBMNCs）. Methods The mononuclear cells were separated from 30 newborns umbilical cord blood. The expressions of nestin and neuron-specific enolase （NSE） were observed by immunofluorescent staining method. HUBMNCs were cultured in vitro, and the changes of the cells morphology were observed with inverted phase contrast microscope. Results There were （2.67±1.29 ） nestin positive cells and （7.37 ±2.46） NSE positive cells in each high power field （ HP, × 200） of HUBMNCs by immunofluoreseent staining. There were 3-5/HP neuron-like cells which were large and with a plurality of coarse long protrusions similar to dendrites and axons at cultured 10th - 12th d. Conclusion There are a few neuron-like cells in HUBMNCs.