主要细胞周期蛋白表达与胃癌临床病理特征的关系

Types of primary cyclins expressed in gastric carcinoma and their relationship with clinicopathologieal features

目的研究主要细胞周期蛋白（cyclins）在胃癌细胞中非时相表达特征，探讨主要cyclins（cyclinD1、cyclinE、cyclinA和cyelinB1）表达与胃癌临床病理特征的关系。方法采用流式细胞术双参数法半定量检测68例胃癌组织中cyclinD1、cyclinE、eyclinA和cyclinB1的表达，并结合患者的临床病理特征进行分析。结果根据不同cyclins在细胞周期中的作用及其异常表达特征，胃癌细胞中主要cyelins表达可分为I型、Ⅱ型、Ⅲ型、Ⅳ型和V型。根据细胞周期内cyclinE、cyclinA和cyclinB1表达特征，Ⅱ型～Ⅳ型分为a亚型、b亚型、c亚型和d亚型。68例胃癌组织中，cyclinsI型、Ⅱ型、Ⅲ型、Ⅳ型和V型表达分别占10．3％（7／68）、22．1％（15／68）、25．0％（17／68）、29．4％（20／68）和13．2％（9／68）。cyclins表达类型与胃癌浸润深度、淋巴结转移程度和TNM分期有关（P〈0．01）。cyelinsI型、Ⅱ型、Ⅲ型、Ⅳ型和V型表达的胃癌淋巴结转移率分别为26．6％、43．8％、82．3％、95．0％和100．0％，1V期患者所占比例分别为0、6．7％、17．6％、25．0％和55．6％，差异有统计学意义（P〈0．01）。cyclins表达亚型与胃癌生长方式、细胞分化程度和Bormann分型相关（P〈0．01）。结论主要cyclins在胃癌的发生、发展及转移过程中有不同形式的异常表达，其不同表达形式与胃癌临床病理特征相关。

Objective To characterize the human primary cyclins （ D1, E, A, B1 ） expressed in gastric carcinoma, and to clarify the relationship between the types of expressed primary eyelins and elinicopathological features of gastric carcinoma. Methods Primary cyclins （ D1, E, A, B1 ） expressed in single cells separated from 68 cases gastric carcinoma tissues were analyzed by flow cytometry. We classified the gastric carcinomas by different types of the expressed primary cyclins, and explore the roles of primary cyclins expressed in cell cycle and the expression patterns of the cyclins. The results were analyzed together with elinieopathologieal features. Results The patterns of expressed primary eyehns could be classified into five types. The proportion was 10.3% （7/68）, 22.1% （15/68）, 25.0% （ 17/68）, 29.4% （20/68）, and 13.2% （9/68）, respectively, from type I to type V. Each type could be, according to the degree of incycle cyclins expressed, divided into different sub-types. The types of primary cyclins expressed were strongly linked to invasive depth and lymph node metastasis of the gastric carcinoma （P 〈 0.01 ）. The rates of lymph node metastasis were 26.6%, 43.8%, 82.3%, 95.0%, and 100.0%, respectively, from type I to type V. The type of primary eyclins expressed was also significantly associated with disease stage （TNM stage）. The proportion of stage 1V disease was 0, 6.7%, 17.6%, 25.0% -1155.6%, respectively, from type I to type V. It was shown that there were relationships between the sub-types of primary cyclins expressed and different growth-types, degree of cell differentiation, or, the tumor gross types （P 〈 0.01 ）.Conclusions The types of primary cyclins expression are different in the process of the occurrence, development and metastasis of gastric carcinoma, and are correlated with clinicopathological features of gastric carcinoma.