摘要
目的:观察大鼠实验性牙周炎牙周纤维病变过程中MCP-1和ICAM-1的表达与分布。方法:选取24只3月龄健康SD大鼠,随机等分为4组(1个正常对照组和3个实验组,每组6只),正常对照组不作任何处理,3个实验组通过丝线结扎上颌左右第二磨牙牙颈部,并配合牙周炎食谱及接种Pg的方式建立牙周炎模型,分别于建模后2(P1)、4(P2)、6(P3)周处死各组大鼠,取实验部位组织块,用免疫组织化学染色法检测MCP-1和ICAM-1的表达与分布。结果:P1、P2、P3各实验组MCP-1和ICAM-1的表达水平均较正常对照组明显增多,差异有显著性(P<0.05)。其中P2组表达水平最高,与其他2组相比有统计学意义(P<0.05);P3组有所下降,与P1相比无明显性差异(P≥0.05)。结论:MCP-1和ICAM-1共同参与牙周纤维组织的炎症过程,其表达随时间呈现一定规律。
AIM ： To observe the expression of MCP-1 and ICAM-1 in periodontal fiber in the rats with ex- perimental periodontitis. METHODS ： 24 SD rats were randomly divided into four groups （ n = 6）. The rats with no treatment served as the controls （ Group N）. The second premolars of other 3 groups were ligated with silk, fed with a periodontitis recipe and inoculated with P. gingivalis. After 2 （ Group P1 ）, 4 （ Group P2） and 6 weeks （ Group P3）, the rats were sacrificed and periodontal tissues were sectioned. Expression and distribution of MCP-1 or ICAM-1 were examined by histoimmunological study. RESULTS ： The expression of MCP-1 and ICAM-1 in the experimental groups increased significantly compared with that in control group （ P 〈 0. 05）. Group P2 showed strongest expression （P 〈 0.05）, while no significant difference of MCP-1 or ICAM-1 expression was found between group P1 and P3 （P≥0.05）. CONCLUSION： These findings suggest that MCP-1 and ICAM-1 might be associated with the patho- genesis of periodontitis.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2013年第2期107-110,共4页
Chinese Journal of Conservative Dentistry
