应用生物发光技术研究甲基硒酸对L9981-Luc肺癌细胞株移植瘤模型生长转移的影响

Influence of MSA on Cell Growth and Spontaneousn Metastasis of L9981-Luc Lung Cancer Transplanted Model in Nude Mice by Bioluminescence Imaging

背景与目的甲基硒酸是一种新型的人工合成的硒化合物。研究发现甲基硒酸对肿瘤细胞的生长转移有抑制作用。本研究的目的是探讨甲基硒酸对L9981-Luc裸鼠异体移植瘤生长和转移能力的抑制作用及机制。方法建立L9981-Luc肺癌细胞株移植瘤模型,用精诺真活体动物可见光成像系统观察肺癌移植瘤肿瘤生长转移情况。实验将6周龄裸鼠15只,随机分为3组,每组5只,对照组每日腹腔注射生理盐水0.2mL;甲基硒酸组每日腹腔注射甲基硒酸溶液50μg(0.2mL);顺铂组每周腹腔注射顺铂4mg/kg。结果接种第21天,不同组间原发瘤发光值比较差异有统计学意义(P=0.002);顺铂组发光值明显低于对照组(P=0.001),甲基硒酸组发光值明显低于对照组(P=0.031)。不同药物处理组胸部转移信号发光值差异无统计学意义(P>0.05)。结论甲基硒酸能明显抑制L9981-Luc裸鼠异体移植瘤生长,并有抑制L9981-Luc原发瘤肺转移的趋势。

Background and objective Methylseleninic acid （MSA） is an artificially developed selenium compound. It has been proven that MSA could inhibit growth and metastasis on many tumor cells. This study investigated whetherMSA has an impact on the growth and metastasis ofL9981-Luc lung cancer transplanted model in nude mice or not. Methods A transplantated tumor model was established in nude mice. Fifteen nude mice were randomly divided into three groups： thecontrol group treated with normal saline （0.2 mL/d）, the MSA group treated with MSA solution （0.2 mL）, and the cisplatin （DDP） group injected intraperitoneally with DDP （4 mg/kg/w）. Inhibition of MSA on tumor growth and tumor metastasiswas observed using the MS Imaging System 200 Series. Results A significant difference was obserced in the primary tumor bioluminescence among the three groups （P=0.002） on 21 days post-inoculation. Primary tumor bioluminescence in theDDP group （P=0.001） and in the MSA group （P=0.031） was significantly lower than that in the control group （P=0.001）. No significant difference in the metastasis bioluminescence of the thoracic area was indicated among the three groups （P〉0.05）.Conclusion MSA can inhibit the growth of planted tumor of transgenic lung cancer cell lines L9981-Luc in nude mice. MSA may also suppress the distant metastasis of the transplanted tumor of transgenic lung cancer cell lines L9981-Luc in nude mice.