生理代谢参数RQ在指导发酵过程优化中的应用

Fermentation Optimization Directed by Physiological Parameter Respiratory Quotient

过程在线参数检测是进行发酵过程工程优化控制的基础。呼吸熵RQ是微生物胞内微观代谢流在宏观代谢参数上的响应,反映了微生物培养过程中底物的利用情况、产物和副产物的合成情况、及微观代谢途径通量的变化。结合发酵葡萄糖酸、2,3-丁二醇谷氨酸、柠檬酸、头孢菌素C、毕赤酵母α-干扰素产品的工业生产过程,分析了以微生物胞内微观代谢与宏观的生理参数RQ为指导的发酵工艺优化策略。RQ值在发酵过程中可以根据尾气数据进行在线采集,对指导通过宏观代谢参数的调控来最优化微生物胞内的代谢途经通量,提高目的产物的产率具有非常重要的意义。

Online measurement of process parameters play a fundamental role in optimization and control of fermentation process engineering. Respiratory quotient （RQ） is the response of intraeellular microscopic flow on global metabolic parameters, which reflect the utilization of substrates, product and by-products synthesis, and the changes of metabolic pathways and flux in microbial cultivation. The optimal controlling strategies of fermentation process based on the combination of parameter RQ in several industrial products production micro-intracellular metabolism and macro-physiological were investigated. RQ, measured by exhaust gas analysis online, is a crucial factor in optimization of the intracellular metabolic pathway flux through the regulation of global metabolic parameters and improvement of the yields of target product.