急性戊型肝炎病毒感染后NK和NKT细胞表达的变化

The changes of NK cell and NKT cell expressions in the infection of acute hepatitis E

目的探讨急性戊型肝炎病毒(HEV)感染后自然杀伤细胞(NK)和自然杀伤T细胞(NKT)的表达变化。方法42例急性HEV感染者分为抗-HEV IgM转阴组(A组,29例)和抗-HEV IgM未转阴组(B组,13例)。采用流式细胞术测定外周血NK和NKT细胞的含量,同时检测肝功能。结果 A组恢复期NK和NKT细胞比例分别为(17.96±7.20)%和(5.05±0.86)%,明显低于急性期的(32.35±10.58)%和(7.89±1.21)%(P<0.05)。B组恢复期NK和NKT细胞比例分别为(26.48±13.98)%和(5.12±0.75)%,均低于急性期的(34.85±11.69)%和(9.63±1.78)%(P<0.05)。HEV感染者的ALT及AST与NK和NKT细胞含量存在明显的正相关(P<0.05)。结论急性HEV感染可以激活NK和NKT细胞,参与机体的免疫反应。

Objective To investigate the changes of natural killer（NK） cell and NK T-cell expressions in the infection of acute hepatitis E.Methods Fourty-two patients with acute hepatitis E were divided into 2 groups of A（29 cases with anti-HEV seroconversion） and B（13 cases without）.The percentages of NK and NK T-cells in serum were examined by fluorescence activated cell sorting（FACS）.The liver functions were measured as well.Results The percentages of NK and NK T-cells in serum in group A were lower in recovery stage than those in acute stage[（17.96±7.20）% and（5.05±0.86）% vs.（32.35±10.58）% and（7.89±1.21）%]（P0.05）,which in group B were similar [（26.48±13.98）% and（5.12±0.75）% vs.（34.85±11.69±）% and（9.63±1.78）%]（P0.05）.The levels of ALT and AST in the patients with acute hepatitis E were positively correlated with the percentages of NK and NK T-cells in serum（P0.05）.Conclusion NK and NKT cells can be activated in the patients with acute hepatitis E,which may play a role in the immune response.However.